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- Question
The melting of 40-45 bp at the oriC site of E. coli requires
Options- A. DNA + ATP + HU
- B. DNA + ATP + HU + DNAb
- C. DNA+ ATP + HU + DNAb + DNAa
- D. DNA + ATP + HU + DNAb + DNAa + Pol III holoenzyme
- Correct Answer
- DNA + ATP + HU
- 1. One hypothesis of DNA replication suggested that the parental DNA molecules are broken into fragments. Both strands of DNA in each of the daughter molecules are made up of an assortment of parental and new DNA. This statement refers to which hypothesis?
Options- A. Conservative theory
- B. Dispersive theory
- C. Semi-conservative theory
- D. Evolutionary theory Discuss
- 2. In a typical electrophoresis experiment, larger fragments of DNA move more
Options- A. slowly, so they appear nearer the top of a gel photograph
- B. quickly, so they appear nearer the top of a gel photograph
- C. slowly, so they appear nearer the bottom of a gel photograph
- D. quickly, so they appear nearer the bottom of a gel photograph Discuss
- 3. Replication in E. coli is initiated by the generation of short RNA primers using
Options- A. RNA polymerase
- B. primase
- C. reverse transcriptase
- D. both (a) and (b) Discuss
- 4. How does ethidium bromide interact with DNA?
Options- A. It binds to adenine
- B. It binds to phosphate
- C. It intercalates between bases
- D. It does not interact Discuss
- 5. Which of the following is correct?
Options- A. Replication starts at a single origin
- B. Replication is bidirectional
- C. Replication is semi conservative
- D. All of the above Discuss
- 6. In DNA replication, one new strand is synthesized complementary to an old (parent) strand and is referred as
Options- A. mutational
- B. semiconservative
- C. discontinuous
- D. antiparallel Discuss
- 7. The role of primase is to
Options- A. dismantle RNA primer
- B. cleave and unwinds short sections of DNA ahead of the replication fork
- C. proofread base pairing
- D. synthesize an RNA primer to begin the elongation process Discuss
- 8. The replication of a linear DNA molecule in a eukaryotic chromosome creates a problem which is reported to be sorted out by
Options- A. using enzyme called telomerase
- B. shortening of the double stranded replicated portion
- C. using RNA as a template
- D. none of the above Discuss
- 9. Which of the following species lack a 'classical' histone H1?
Options- A. Saccharomyces cerevisiae
- B. Strongylocentrotus purpuratus
- C. Xenopus laevis
- D. Arabidopsis thaliana Discuss
- 10. The enzyme responsible for breaking the hydrogen bonds, and thus separating the DNA strands during DNA synthesis is
Options- A. DNA polymerase
- B. strandase
- C. helicase
- D. none of these Discuss
DNA Structure and Replication problems
Search Results
Correct Answer: Dispersive theory
Correct Answer: slowly, so they appear nearer the top of a gel photograph
Correct Answer: both (a) and (b)
Correct Answer: It intercalates between bases
Correct Answer: All of the above
Correct Answer: semiconservative
Correct Answer: synthesize an RNA primer to begin the elongation process
Correct Answer: using enzyme called telomerase
Correct Answer: Saccharomyces cerevisiae
Correct Answer: helicase
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