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Home Biochemistry DNA Structure and Replication Comments

  • Question
  • How does ethidium bromide interact with DNA?


  • Options
  • A. It binds to adenine
  • B. It binds to phosphate
  • C. It intercalates between bases
  • D. It does not interact

  • Correct Answer
  • It intercalates between bases 


  • DNA Structure and Replication problems


    Search Results


    • 1. Which of the following is correct?

    • Options
    • A. Replication starts at a single origin
    • B. Replication is bidirectional
    • C. Replication is semi conservative
    • D. All of the above
    • Discuss
    • 2. After one replication in 15N, how many bands should be observed in a cesium chloride gradient?

    • Options
    • A. One
    • B. Two
    • C. Three
    • D. A smear
    • Discuss
    • 3. DNA polymerases I also has

    • Options
    • A. 5'-3' exonuclease activity
    • B. 3'-5'exonuclease activity
    • C. both (a) and (b)
    • D. 5'-3' endonuclease activity
    • Discuss
    • 4. Nucleosome is

    • Options
    • A. an octamer of four histones with H1 protein
    • B. an hexamer of three histones with H1 protein
    • C. an hexamer of three histones without H1 protein
    • D. an octamer of four histones without H1 protein
    • Discuss
    • 5. Number of times the two DNA strands are interwined in circular DNA and is known as

    • Options
    • A. helix frequency
    • B. linking number
    • C. twisting number
    • D. none of these
    • Discuss
    • 6. Replication in E. coli is initiated by the generation of short RNA primers using

    • Options
    • A. RNA polymerase
    • B. primase
    • C. reverse transcriptase
    • D. both (a) and (b)
    • Discuss
    • 7. In a typical electrophoresis experiment, larger fragments of DNA move more

    • Options
    • A. slowly, so they appear nearer the top of a gel photograph
    • B. quickly, so they appear nearer the top of a gel photograph
    • C. slowly, so they appear nearer the bottom of a gel photograph
    • D. quickly, so they appear nearer the bottom of a gel photograph
    • Discuss
    • 8. One hypothesis of DNA replication suggested that the parental DNA molecules are broken into fragments. Both strands of DNA in each of the daughter molecules are made up of an assortment of parental and new DNA. This statement refers to which hypothesis?

    • Options
    • A. Conservative theory
    • B. Dispersive theory
    • C. Semi-conservative theory
    • D. Evolutionary theory
    • Discuss
    • 9. The melting of 40-45 bp at the oriC site of E. coli requires

    • Options
    • A. DNA + ATP + HU
    • B. DNA + ATP + HU + DNAb
    • C. DNA+ ATP + HU + DNAb + DNAa
    • D. DNA + ATP + HU + DNAb + DNAa + Pol III holoenzyme
    • Discuss
    • 10. In DNA replication, one new strand is synthesized complementary to an old (parent) strand and is referred as

    • Options
    • A. mutational
    • B. semiconservative
    • C. discontinuous
    • D. antiparallel
    • Discuss


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