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- Question
Which of the following is used to determine the appropriate stringency (Wallace rule)?
Options- A. Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair)
- B. Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair)
- C. Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)
- D. Tm = 69.3° + 0.41 (%)G+C -650/1
- Correct Answer
- Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)
- 1. Which of the following is an active site on its single polypeptide chain for DNA Polymerase I?
Options- A. 5' ? 3' polymerase
- B. 3' ? 5' exonuclease
- C. 5' ? 3' exonuclease
- D. all of the above Discuss
- 2. The size of a linear DNA fragment in a gel can be estimated by
Options- A. fluorescence in UV light
- B. the sedimentation coefficient
- C. the time it takes the gel to run
- D. comparison with a molecular weight standard such as Hind III digested phage lambda Discuss
- 3. An enzyme that recognizes foreign DNA, and results in a cut in that DNA is called
Options- A. transposase
- B. helicase
- C. reverse transcriptase
- D. restriction endonuclease Discuss
- 4. There is a single origin for DNA replication in bacteria. How many origins of replication are found in the much larger genomes of eukaryotes such as mammals?
Options- A. One
- B. About 100
- C. Thousands and tens of thousands
- D. none of these Discuss
- 5. What is the IUPAC code for an A, C, T ambiguity in a nucleic acid sequence?
Options- A. B
- B. H
- C. S
- D. None of these Discuss
- 6. Automated DNA sequencing uses the chain termination method
Options- A. but with an oligonucleotide primer labeled with a fluorescent dye
- B. but with a polynucleotide primer labeled with a fluorescent dye
- C. with no primer
- D. either (b) or (c) Discuss
- 7. Number of times the two DNA strands are interwined in circular DNA and is known as
Options- A. helix frequency
- B. linking number
- C. twisting number
- D. none of these Discuss
- 8. Nucleosome is
Options- A. an octamer of four histones with H1 protein
- B. an hexamer of three histones with H1 protein
- C. an hexamer of three histones without H1 protein
- D. an octamer of four histones without H1 protein Discuss
- 9. DNA polymerases I also has
Options- A. 5'-3' exonuclease activity
- B. 3'-5'exonuclease activity
- C. both (a) and (b)
- D. 5'-3' endonuclease activity Discuss
- 10. After one replication in 15N, how many bands should be observed in a cesium chloride gradient?
Options- A. One
- B. Two
- C. Three
- D. A smear Discuss
DNA Structure and Replication problems
Search Results
Correct Answer: 5' ? 3' polymerase
Correct Answer: comparison with a molecular weight standard such as Hind III digested phage lambda
Correct Answer: restriction endonuclease
Correct Answer: Thousands and tens of thousands
Correct Answer: H
Correct Answer: but with an oligonucleotide primer labeled with a fluorescent dye
Correct Answer: linking number
Correct Answer: an octamer of four histones without H1 protein
Correct Answer: 3'-5'exonuclease activity
Correct Answer: One
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