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Home Biochemistry Gas Chromatography Comments

  • Question
  • Doubling the column's length increases resolution by a factor of


  • Options
  • A. (2)0.5
  • B. 2
  • C. 3
  • D. 4

  • Correct Answer
  • (2)0.5 


  • Gas Chromatography problems


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    • 1. Helium is generally preferred as carrier gas over nitrogen and hydrogen because

    • Options
    • A. it is inert
    • B. it has a lower viscosity
    • C. it doubles up as a party gas for balloons and funny voices
    • D. all of above
    • Discuss
    • 2. What does the selectivity factor describe?

    • Options
    • A. The proportional difference in widths of two chromatographic peaks
    • B. The maximum number of different species which a column can separate simultaneously
    • C. The relative separation achieved between two species
    • D. None of the above
    • Discuss
    • 3. Column bleeding occurs when

    • Options
    • A. elution of the analyte is extended over time
    • B. the column is cracked and stationary phase leaks out
    • C. traces of the stationary phase are eluted
    • D. the column breaks during installation and causes personal injury
    • Discuss
    • 4. Which of these effects result from slow injection of a large sample volume?

    • Options
    • A. Increased resolution
    • B. Decreased resolution
    • C. Non-linear detector response
    • D. Constant resolution
    • Discuss
    • 5. Which of the following is not used for detection in GC?

    • Options
    • A. Infrared spectroscopy
    • B. NMR
    • C. Flame ionisation
    • D. Electrical conductivity
    • Discuss
    • 6. The DNA polymerase in bacteria, responsible for DNA synthesis is

    • Options
    • A. Polymerase I
    • B. Polymerase II
    • C. Polymerase III
    • D. all of these
    • Discuss
    • 7. Which of the following are short nucleotides strands made by DNA polymerase working in the opposite direction of the replication fork?

    • Options
    • A. Leading strands
    • B. Okazaki fragments
    • C. Primers
    • D. Lagging strand
    • Discuss
    • 8. DNA replication results in

    • Options
    • A. two completely new DNA molecules
    • B. two DNA molecules such that each one contains a strand of the original
    • C. one new DNA molecules 1 old molecule
    • D. one new molecule of RNA
    • Discuss
    • 9. Which of these drugs bind to DNA and cuts it, producing hydroxyl radicals?

    • Options
    • A. Bleomycin
    • B. Erythromycin
    • C. Tunicamycin
    • D. All of these
    • Discuss
    • 10. The RNA primers used to initiate replication in E. coli

    • Options
    • A. result in Okazaki fragments on leading strand
    • B. are removed by Pol I
    • C. are joined together by DNA ligase
    • D. are removed by helicase + ATP
    • Discuss


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