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Home Biochemistry Recombinant DNA Technology Comments

  • Question
  • Knockout mice are created by


  • Options
  • A. mutagenizing a mouse and selecting for mutant offspring
  • B. creating a chimera by fusing cells from two different cell lines
  • C. infecting the mouse with a retrovirus
  • D. transfecting embryonic stem cells with an altered gene sequence

  • Correct Answer
  • transfecting embryonic stem cells with an altered gene sequence 


  • Recombinant DNA Technology problems


    Search Results


    • 1. A plasmid

    • Options
    • A. is a circular DNA molecule
    • B. always contains an origin of replication
    • C. usually contains one or more restriction sites
    • D. all of the above
    • Discuss
    • 2. Which of the following is not true of small inbreeding populations?

    • Options
    • A. They tend to loose genetic diversity
    • B. There is an increased incidence of recessive diseases
    • C. Alleles may become fixed
    • D. Mutation is increased
    • Discuss
    • 3. Restriction enzymes

    • Options
    • A. protect bacteria from viral infection
    • B. cut DNA in a staggered fashion
    • C. cut DNAs producing a blunt end
    • D. all of the above
    • Discuss
    • 4. What enzyme forms covalent bonds between restriction fragments?

    • Options
    • A. DNA primase
    • B. DNA helicase
    • C. DNA polymerase
    • D. DNA ligase
    • Discuss
    • 5. When populations are small, gene frequencies can change from generation to generation and some alleles may become fixed in a population. This is called __________ .

    • Options
    • A. assortative mating
    • B. inbreeding
    • C. heterosis
    • D. genetic drift
    • Discuss
    • 6. Which of these restriction enzymes produce blunt ends?

    • Options
    • A. SaII
    • B. EcoRV
    • C. XhoI
    • D. HindIII
    • Discuss
    • 7. A human cDNA library

    • Options
    • A. contains DNA for specific human proteins
    • B. contains DNA for virtually all of the human proteins in vectors
    • C. cannot be used to obtain human genes because it would be radioactive
    • D. none of the above
    • Discuss
    • 8. Gluconeogenesis uses

    • Options
    • A. 3 ATPs and 2 GTPs per glucose
    • B. 2 ATPs and 1 GTPs per glucose
    • C. 3 ATPs and 3 GTPs per glucose
    • D. 4 ATPs and 2 GTPs per glucose
    • Discuss
    • 9. Citric acid accumulation would

    • Options
    • A. stimulate phosphofructokinase activity
    • B. stimulate fructose 1,6 diphosphatase activity
    • C. inhibit phosphofructokinase activity
    • D. both (b) and (c)
    • Discuss
    • 10. Hydrolysis of lactose yields

    • Options
    • A. galactose and fructose
    • B. galactose and glucose
    • C. glucose and fructose
    • D. fructose and galactose
    • Discuss


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