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Home Biochemistry Recombinant DNA Technology Comments

  • Question
  • The unpaired nucleotides produced by the action of restriction enzymes are referred to have


  • Options
  • A. sticky ends
  • B. single strands
  • C. restriction fragments
  • D. ligases

  • Correct Answer
  • sticky ends 


  • Recombinant DNA Technology problems


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    • 1. Which type of restriction enzymes do not usually require ATP?

    • Options
    • A. Type I
    • B. Type II
    • C. Type III
    • D. Type IV
    • Discuss
    • 2. Why is golden rice pale yellow in color?

    • Options
    • A. It is rich in chlorophyll a.
    • B. It is rich in beta-carotene.
    • C. It is rich in chlorophyll b.
    • D. It is rich in phycobilins.
    • Discuss
    • 3. Which of these genes codes for a protein that plays a role in growth?

    • Options
    • A. DCP1
    • B. SCLC6A4
    • C. KRTHA1
    • D. GH1
    • Discuss
    • 4. Which of the following pair will produce complementary sticky ends?

    • Options
    • A. Eco RI & MspI
    • B. Msp I & HPA II
    • C. Sau 3A & Bam HI
    • D. Mbo I & Sau 3A
    • Discuss
    • 5. Restriction enzymes are named for

    • Options
    • A. the person who discovered
    • B. the bacterium they are derived from
    • C. the viral DNA that they attack
    • D. none of the above
    • Discuss
    • 6. The RP13 gene of chromosome 17 codes for a protein __________ .

    • Options
    • A. involved in glucose transport
    • B. that is a component of hair and nails
    • C. involved in eye development
    • D. involved in the determination of personality
    • Discuss
    • 7. The order for the construction of a cDNA fragment from mRNA is to

    • Options
    • A. bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase
    • B. treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
    • C. digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
    • D. bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase
    • Discuss
    • 8. Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because

    • Options
    • A. in both cases molecules migrate to the anode
    • B. both techniques rely on a constant charge to mass ratio
    • C. both techniques utilize the sieving properties of gels
    • D. all of the above
    • Discuss
    • 9. X-rays cause

    • Options
    • A. the formation of thymine dimers
    • B. ionization of water in the cell
    • C. heat
    • D. none of the above
    • Discuss
    • 10. Under which of the following conditions would population gene frequencies remain the same?

    • Options
    • A. Selection for homozygotes
    • B. Small population size
    • C. Active migration between groups
    • D. Random mating
    • Discuss


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