5. Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are
Options
A. copies of the gene with an altered sequence so that a restriction site is inserted
B. mutagenized copies of a gene
C. short sequences that will hybridize only to a specific base sequence
D. PCR-amplified variable numbers of tandem repeats (VNTRs)
Correct Answer: bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase