Discussion
Home ‣ Biochemistry ‣ Recombinant DNA Technology Comments
- Question
First discovered, Type II restriction endonuclease was
Options- A. Hinf I
- B. Eco K
- C. Hind II
- D. EcoRI
- Correct Answer
- Hind II
- 1. Which of the following genetic diseases would be amenable to genetic engineering?
Options- A. Down's syndrome
- B. Muscular dystrophy
- C. Cystic fibrosis
- D. Cri du Chat Discuss
- 2. Isoschizomers recognize
Options- A. same recognition sequence but different recognition site
- B. same recognition site and recognition sequence
- C. same recognition site and different recognition sequence
- D. different recognition site and different recognition sequence Discuss
- 3. Which of the following techniques can be used to determine the defective gene and for developing cancer?
Options- A. Western blot
- B. Southern blot
- C. Northern blot
- D. Eastern blot Discuss
- 4. In gel electrophoresis, DNA molecules migrate from __________ to __________ ends of the gel.
Options- A. negative ... positive
- B. basic ... acidic
- C. long ... short
- D. positive to negative Discuss
- 5. In order to insert a foreign gene into a plasmid, both must __________
Options- A. have identical DNA sequences
- B. originate from the same type of cell
- C. be cut by the same restriction enzyme
- D. be of the same length Discuss
- 6. Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are
Options- A. copies of the gene with an altered sequence so that a restriction site is inserted
- B. mutagenized copies of a gene
- C. short sequences that will hybridize only to a specific base sequence
- D. PCR-amplified variable numbers of tandem repeats (VNTRs) Discuss
- 7. Which of the following can be diagnosed by amniocentesis?
Options- A. Down's syndrome
- B. Cystic Fibrosis
- C. Sickle cell anemia
- D. All of these Discuss
- 8. The transfer of antibiotic-resistant genes from genetically engineered bacteria to disease-causing bacteria __________ .
Options- A. would be of no concern if it occurred.
- B. has occurred
- C. can never occur
- D. seems unlikely Discuss
- 9. Restriction enzymes are named for
Options- A. the person who discovered
- B. the bacterium they are derived from
- C. the viral DNA that they attack
- D. none of the above Discuss
- 10. Which of the following pair will produce complementary sticky ends?
Options- A. Eco RI & MspI
- B. Msp I & HPA II
- C. Sau 3A & Bam HI
- D. Mbo I & Sau 3A Discuss
Recombinant DNA Technology problems
Search Results
Correct Answer: Cystic fibrosis
Correct Answer: same recognition site and recognition sequence
Correct Answer: Southern blot
Correct Answer: negative ... positive
Correct Answer: be cut by the same restriction enzyme
Correct Answer: short sequences that will hybridize only to a specific base sequence
Correct Answer: All of these
Correct Answer: seems unlikely
Correct Answer: the bacterium they are derived from
Correct Answer: Sau 3A & Bam HI
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