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- Question
In a gel filtration column
Options- A. smaller proteins enter the beads more readily
- B. large proteins elute first
- C. both (a) and (b)
- D. large proteins enter the beads more readily
- Correct Answer
- both (a) and (b)
- 1. In isoelectric focusing, proteins are separated on the basis of their
Options- A. relative content of positively charged residue only
- B. relative content of negatively charged residue only
- C. size
- D. relative content of positively and negatively charged residue Discuss
- 2. The subunit molecular weight as well as the number of subunits in the quaternary structure can be determined by
Options- A. SDS-PAGE electrophoresis
- B. gel filtration chromatography
- C. combining information from (a)and (b)
- D. isoelectric focusing Discuss
- 3. In an SDS-PAGE
Options- A. proteins are denatured by the SDS
- B. proteins have the same charge-to-mass ratio
- C. smaller proteins migrate more rapidly through the gel
- D. all of the above Discuss
- 4. Proteins are separated in an SDS-PAGE experiment on the basis of their
Options- A. positively charged side chains
- B. molecular weight
- C. negatively charged side chains
- D. different isoelectric points Discuss
- 5. Electrophoresis of histones and myoglobin under non-denaturing conditions (pH = 7.0) results in
Options- A. both proteins migrate to the anode
- B. histones migrate to the anode and myoglobin migrates to the cathode
- C. histones migrate to the cathode and myoglobin migrates to the anode
- D. both proteins migrate to the cathode Discuss
- 6. Proteins can be visualized directly in gels by
Options- A. staining them with the dye
- B. using electron microscope only
- C. measuring their molecular weight
- D. none of these Discuss
- 7. The nucleophile in serine proteases is
Options- A. Serine
- B. water
- C. both (a) and (b)
- D. Asparagine Discuss
- 8. Enzyme-driven metabolic pathways can be made more efficient by
Options- A. concentrating enzymes within specific cellular compartments
- B. grouping enzymes into free-floating, multienzyme complexes
- C. fixing enzymes into membranes so that they are adjacent to each other
- D. All of the above Discuss
- 9. In the enzyme-catalyzed reaction shown below, what will be the effect on substances A, B, C, and D of inactivating the enzyme labeled E2?
A ---(E1)---> B ---(E2)---> C ---(E3)--->
Options- A. A, B, C, and D will all still be produced
- B. A, B, and C will still be produced, but not D
- C. A and B will still be produced, but not C or D
- D. A will still be produced, but not B, C, or D Discuss
- 10. Before they can react, many molecules need to be destabilized. This state is typically achieved through
Options- A. changing the three-dimensional shape of the molecule
- B. oxidizing the molecules by removing electrons
- C. changing the reaction from a biosynthetic to a catabolic pathway
- D. the input of a small amount of activation energy Discuss
Gel Electrophoresis problems
Search Results
Correct Answer: relative content of positively and negatively charged residue
Correct Answer: combining information from (a)and (b)
Correct Answer: all of the above
Correct Answer: molecular weight
Correct Answer: histones migrate to the cathode and myoglobin migrates to the anode
Correct Answer: staining them with the dye
Correct Answer: both (a) and (b)
Correct Answer: All of the above
Correct Answer: A and B will still be produced, but not C or D
Correct Answer: the input of a small amount of activation energy
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