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Biochemistry
‣
Polymerase Chain Reaction
Comments
Question
DNA fragments in a restriction digest can be separated by electrophoresis in
Options
A. poly acrylamide
B. agarose gel
C. both (a) and (b)
D. none of these
Correct Answer
both (a) and (b)
Polymerase Chain Reaction problems
Search Results
1. PCR is an abbreviation for
Options
A. Protein Change Reaction
B. Polymerase Chain Reaction
C. Positive Charge Reaction:
D. Preliminary Change Rate
Show Answer
Scratch Pad
Discuss
Correct Answer: Polymerase Chain Reaction
2. How many ds DNA molecule can be produced, which comprise precisely the target region in double strand form during 4th cycle of PCR?
Options
A. Two ds DNA molecule
B. Three ds DNA molecule
C. Eight ds DNA molecule
D. Four ds DNA molecule
Show Answer
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Discuss
Correct Answer: Four ds DNA molecule
3. PCR can be used in
Options
A. cloning
B. sequencing
C. medical diagnosis and forensic medicine
D. all of the above
Show Answer
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Discuss
Correct Answer: all of the above
4. Transformation means
Options
A. formation of a pilus
B. acquiring DNA from the bacterial cell environment
C. plasmid containing a F factor
D. F+ and F- strains of bacteria
Show Answer
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Discuss
Correct Answer: acquiring DNA from the bacterial cell environment
5. PCR amplifies millions of copies of
Options
A. a short fragment of DNA
B. the entire genome (all the DNA in a sample)
C. both (a) and (b)
D. short chain RNA
Show Answer
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Correct Answer: a short fragment of DNA
6. Northern blotting is
Options
A. widely different than southern blotting
B. another name for southern blotting
C. analogous to southern blotting
D. none of the above
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Correct Answer: analogous to southern blotting
7. For gene transfer to be effective, transforming DNA must be
Options
A. incorporated into the bacterial chromosome
B. incorporated into a viral genome
C. free in the bacterial cytoplasm
D. none of the above
Show Answer
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Correct Answer: incorporated into the bacterial chromosome
8. Bacteriophages are
Options
A. cells in the blood that eat bacteria
B. a class of bacteria
C. bacterial viruses
D. none of the above
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Correct Answer: bacterial viruses
9. The PCR, polymerase chain reaction is becoming the method of choice for
Options
A. Alteration of gene
B. screening gene
C. sterilization of gene
D. all of these
Show Answer
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Discuss
Correct Answer: screening gene
10. During glycolysis, the major energy generating step involves
Options
A. pyruvate kinase
B. phosphoglycerate kinase
C. glyceraldehyde-3 -dehydrogenase
D. Phosphofructokinase
Show Answer
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Discuss
Correct Answer: glyceraldehyde-3 -dehydrogenase
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