Discussion
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- Question
PCR can be used in
Options- A. cloning
- B. sequencing
- C. medical diagnosis and forensic medicine
- D. all of the above
- Correct Answer
- all of the above
- 1. Transformation means
Options- A. formation of a pilus
- B. acquiring DNA from the bacterial cell environment
- C. plasmid containing a F factor
- D. F+ and F- strains of bacteria Discuss
- 2. PCR amplifies millions of copies of
Options- A. a short fragment of DNA
- B. the entire genome (all the DNA in a sample)
- C. both (a) and (b)
- D. short chain RNA Discuss
- 3. The viruses that can achieve neoplastic transformation are called
Options- A. DNA tumor viruses
- B. RNA tumor viruses
- C. retroviruses HIV
- D. none of these Discuss
- 4. A PCR cycle consists of
Options- A. three steps, denaturation, primer annealing and elongation
- B. three steps, denaturation, initiation and elongation
- C. three steps, primer annealing, elongation and termination
- D. three steps, initiation, elongation and termination Discuss
- 5. Restriction maps
Options- A. allows comparison between DNA molecules without the need to determine nucleotide sequence
- B. allows comparison between DNA molecules but requires to determine nucleotide sequence
- C. does not allow comparison between DNA molecules
- D. none of the above Discuss
- 6. How many ds DNA molecule can be produced, which comprise precisely the target region in double strand form during 4th cycle of PCR?
Options- A. Two ds DNA molecule
- B. Three ds DNA molecule
- C. Eight ds DNA molecule
- D. Four ds DNA molecule Discuss
- 7. PCR is an abbreviation for
Options- A. Protein Change Reaction
- B. Polymerase Chain Reaction
- C. Positive Charge Reaction:
- D. Preliminary Change Rate Discuss
- 8. DNA fragments in a restriction digest can be separated by electrophoresis in
Options- A. poly acrylamide
- B. agarose gel
- C. both (a) and (b)
- D. none of these Discuss
- 9. Northern blotting is
Options- A. widely different than southern blotting
- B. another name for southern blotting
- C. analogous to southern blotting
- D. none of the above Discuss
- 10. For gene transfer to be effective, transforming DNA must be
Options- A. incorporated into the bacterial chromosome
- B. incorporated into a viral genome
- C. free in the bacterial cytoplasm
- D. none of the above Discuss
Polymerase Chain Reaction problems
Search Results
Correct Answer: acquiring DNA from the bacterial cell environment
Correct Answer: a short fragment of DNA
Correct Answer: DNA tumor viruses
Correct Answer: three steps, denaturation, primer annealing and elongation
Correct Answer: allows comparison between DNA molecules without the need to determine nucleotide sequence
Correct Answer: Four ds DNA molecule
Correct Answer: Polymerase Chain Reaction
Correct Answer: both (a) and (b)
Correct Answer: analogous to southern blotting
Correct Answer: incorporated into the bacterial chromosome
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