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- Question
The IPTG is a gratuitous inducers of (?-galactosidase, and x-gal is a chromogenic substrate. When ?-complementation occurs which colour is produced?
Options- A. Blue
- B. White
- C. Brown
- D. No colour is produced
- Correct Answer
- Blue
- 1. Radial Immunodiffusion is similar to
Options- A. double-diffusion
- B. gel diffusion
- C. Ouchterloney technique
- D. all of these Discuss
- 2. Which of the following radioactive element is generally involved in RIA?
Options- A. Tritium
- B. Carbon-14
- C. Iodine-125
- D. All of these Discuss
- 3. Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectively
Options- A. 2000 kb and 20kb DNA
- B. 1000 kb and l0kb DNA
- C. 20 kb and 2000 kb DNA
- D. 10 kb and 1000 kb DNA Discuss
- 4. Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?
Options- A. Open circular, super coiled, linear plasmid DNA
- B. Linear plasmid, open circular, super coiled plasmid
- C. Open circular, super coiled, linear plasmid DNA
- D. Open circular, linear plasmid, super coiled plasmid Discuss
- 5. What is the ELISA test intended to measure?
Options- A. Antibody to HIV only
- B. Antigen to HIV only
- C. Presence of free, circulating virus in the patient
- D. Antibodies directed against HLA molecules Discuss
- 6. Which of the following is an immunodiffusion test?
Options- A. Double-diffusion
- B. Gel diffusion
- C. Ouchterloney technique
- D. All of these Discuss
- 7. An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that
Options- A. there was more than one antigen
- B. the antibody was impure
- C. the temperature was too high
- D. there was one antibody and one antigen Discuss
- 8. Which of the following is an immunoelectrophoresis technique?
Options- A. Two-dimensional immunoelectrophoresis
- B. Counterimmunoelectrophoresis
- C. Coimmunoelectrophoresis
- D. Both (a) and (b) Discuss
- 9. Immunoaffinity chromatography can be used in biochemical applications to
Options- A. break down antibody structure
- B. purify protein antigen
- C. break down antigen and analyze quantitatively
- D. none of the above Discuss
- 10. What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?
Options- A. The ELISA would not develop when the substrate was added
- B. The ELISA would develop normally
- C. All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
- D. None of the above Discuss
Immunological Techniques problems
Search Results
Correct Answer: double-diffusion
Correct Answer: All of these
Correct Answer: 20 kb and 2000 kb DNA
Correct Answer: Linear plasmid, open circular, super coiled plasmid
Correct Answer: Antibody to HIV only
Correct Answer: All of these
Correct Answer: there was more than one antigen
Correct Answer: Both (a) and (b)
Correct Answer: purify protein antigen
Correct Answer: All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
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