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Biochemistry
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Protein Purification
See What Others Are Saying!
Question
During successful purification scheme, this may be expected that the
Options
A. specific activity increases
B. specific activity decreases
C. number of proteins in the sample decreases
D. both (a) and (c)
Correct Answer
both (a) and (c)
More questions
1. Transcription begins
Options
A. at the start codon
B. upstream from the start codon
C. downstream from the start codon
D. none of these
Show Answer
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Discuss
Correct Answer: upstream from the start codon
2. Vibrational spectroscopy is
Options
A. a large mass on a weak spring
B. a flashlight through a prism and shake it
C. a class of spectroscopic techniques which analyzes molecular motions
D. an Infrared spectroscopy
Show Answer
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Correct Answer: a class of spectroscopic techniques which analyzes molecular motions
3. DNA replication takes place in which direction?
Options
A. 3' to 5'
B. 5 'to 3'
C. Randomly
D. Vary from organism to organism
Show Answer
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Discuss
Correct Answer: 5 'to 3'
4. Sex lethal (sxl) in Drosophila is transcribed when
Options
A. the X autosome balance is less than 0.5
B. the X autosome balance is 1 or greater
C. the X autosome balance equals 0.5
D. none of the above
Show Answer
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Correct Answer: the X autosome balance is 1 or greater
5. Type I proteins (plasma membrane) have a
Options
A. cleavage N- terminal signal sequence and a hydrophobic stop transfer sequence
B. cleavaged N- terminal signal sequence that doubles as the membrane anchoring sequence
C. multiple signal sequence and a hydrophobic stop transfer sequence
D. multiple signal sequence that doubles as the membrane anchoring sequence
Show Answer
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Discuss
Correct Answer: cleavage N- terminal signal sequence and a hydrophobic stop transfer sequence
6. Amino acids are joined together into a protein chain by which of the following?
Options
A. Transfer RNA
B. DNA polymerase
C. Hydrogen bonds
D. Messenger RNA
Show Answer
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Discuss
Correct Answer: Transfer RNA
7. What is the ELISA test intended to measure?
Options
A. Antibody to HIV only
B. Antigen to HIV only
C. Presence of free, circulating virus in the patient
D. Antibodies directed against HLA molecules
Show Answer
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Discuss
Correct Answer: Antibody to HIV only
8. The eluent strength is a measure of
Options
A. solvent adsorption energy
B. solvent absorption energy
C. solvent diffusivity
D. solvent mixing index
Show Answer
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Discuss
Correct Answer: solvent adsorption energy
9. What would happen if serum is omitted from the ELISA, but all other steps remain same?
Options
A. Anti-human Ig-conjugate would not bind and be washed away
B. The OD values would be nearly the same as the assay control
C. Both (a) and (b)
D. Anti-human Ig-conjugate would bind non-specifically to the ELISA plate
Show Answer
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Discuss
Correct Answer: Both (a) and (b)
10. In reversed phase HPLC, there is a
Options
A. non polar solvent/polar column
B. polar solvent/non-polar column
C. non polar solvent/non-polar column
D. any of the above
Show Answer
Scratch Pad
Discuss
Correct Answer: polar solvent/non-polar column
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More in Biochemistry:
Allosteric Effects
Amino Acid Metabolism
Anti Bodies
Antigen
ATP Synthesis and Fatty Acid Oxidation
Carbohydrate
Cell Signalling and Transduction
Cell Structure and Compartments
Chromatography
Disease Associated with Immune System
DNA Structure and Replication
Enzymes
FT IR Spectroscopy
Gas Chromatography
Gel Electrophoresis
Genetic Code and Regulation
Genetic Regulation Prokaryotes
Glycolysis
HPLC
Immune Response
Immune System
Immunological Techniques
Lipid
Membrane Structure and Functions
Minerals
Nitrogen Metabolism
NMR Spectroscopy
Nucleic Acids
Oxidative Phosphorylation
Photosynthesis and Respiration
Polymerase Chain Reaction
Protein and Nucleic Acid Interactions
Protein Purification
Protein Stability
Protein Structure
Protein Synthesis
Recombinant DNA Technology
RNA Structure
Spectroscopy
Structure and Properties of Amino Acids
Structure and Properties of Peptides
TCA Cycle
Thermodynamics and Free Energy
Transcription and Regulation
UV Luminance Spectroscopy
Vitamins and Coenzymes
Water, pH and Macromolecules