Recombinant DNA problems
- 1. An animal, that has gained new genetic information from the acquisition of foreign DNA, is considered as
Options- A. a chimera
- B. a transgenic animal
- C. a vector
- D. an enzyme that links DNA molecules Discuss
Correct Answer: a transgenic animal
- 2. A short molecule containing 2-20 nucleotide is
Options- A. plasmid
- B. vector
- C. oligonucleotide
- D. mononucleotide Discuss
Correct Answer: oligonucleotide
- 3. A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as
Options- A. southern blotting
- B. northern blotting
- C. polymerase chain reaction
- D. DNA replication Discuss
Correct Answer: polymerase chain reaction
- 4. The Southern blotting technique depends on
Options- A. similarities between the sequences of probe DNA and experimental DNA
- B. similarities between the sequences of probe RNA and experimental RNA
- C. similarities between the sequences of probe protein and experimental protein
- D. the molecular mass of proteins Discuss
Correct Answer: similarities between the sequences of probe DNA and experimental DNA
- 5. In genetic engineering, a chimera is
Options- A. an enzyme that links DNA molecules
- B. a plasmid that contains foreign DNA
- C. a virus that infects bacteria
- D. a fungi Discuss
Correct Answer: a plasmid that contains foreign DNA
- 6. Which of the following vector can maintain the largest fragment of foreign DNA?
Options- A. YAC
- B. Cosmid
- C. Plasmid
- D. Phage Discuss
Correct Answer: YAC
- 7. The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of
Options- A. genetic engineering
- B. population genetics
- C. microbiology
- D. protein engineering Discuss
Correct Answer: genetic engineering
- 8. What is the normal role of restriction endonucleases in bacterial cells?
Options- A. To degrade the bacterial chromosome into small pieces during replication
- B. To degrade invading phage DNA
- C. To produce RNA primers for replication
- D. All of the above Discuss
Correct Answer: To degrade invading phage DNA
- 9. The advantage of using DNA polymerases from thermophilic organisms in PCR is that
Options- A. the DNA polymerases of these bacteria are much faster than those from other organisms
- B. the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands
- C. the DNA polymerases of these bacteria never make mistakes while replicating DNA
- D. all of the above Discuss
Correct Answer: the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands
- 10. For gene probes to be useful they must
Options- A. be large enough to contain gene-specific sequences
- B. be labeled in some manner to allow detection
- C. both (a) and (b)
- D. none of the above Discuss
Correct Answer: both (a) and (b)
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