Gene Transfer in Plants problems
- 1. Integrated octopine T DNA occurs as
Options- A. single segment
- B. two segments
- C. three segments
- D. four segments Discuss
Correct Answer: two segments
- 2. Crown gall tissue
Options- A. can be cultivated in vitro in absence of bacteria
- B. retains tumorous properties when cultivated
- C. both (a) and (b)
- D. shows tumorous properties only in presence of bacteria Discuss
Correct Answer: both (a) and (b)
- 3. Vir genes required for the T-DNA transfer and processing are located
Options- A. on the T-DNA
- B. outside the T-DNA region
- C. on the plant genome
- D. none of these Discuss
Correct Answer: outside the T-DNA region
- 4. Virulence trait of Agrobacterium tumefaciens is borne on
Options- A. chromosomal DNA
- B. tumour inducing plasmid DNA
- C. both chromosomal and plasmid DNA
- D. cryptic plasmid DNA Discuss
Correct Answer: tumour inducing plasmid DNA
- 5. Which of the following are used as selection marker for the cells transformed with Agrobacterium?
Options- A. Neomycin phosphotransferase
- B. Streptomycin phosphotransferase
- C. Hygromycin phosphotransferase
- D. any of the above Discuss
Correct Answer: any of the above
- 6. The right segment of octopine T-DNA (TR) is necessary for
Options- A. enzymes for agropine biosynthesis
- B. tumour formation
- C. conjugative transfer
- D. all of these Discuss
Correct Answer: enzymes for agropine biosynthesis
- 7. Opines that are present in crown gall tumour include
Options- A. octopine
- B. nopaline
- C. agropine
- D. all of these Discuss
Correct Answer: all of these
- 8. Which technique is used to introduce genes into dicots?
Options- A. electroporation
- B. particle acceleration
- C. microinjection
- D. Ti plasmid infection Discuss
Correct Answer: Ti plasmid infection
- 9. Which of the following is true about Agrobacterium tumefaciens?
Options- A. It causes crown gall disease of plants
- B. It infects gymosperms
- C. It infects dicotyledonous angiosperms
- D. All of the above Discuss
Correct Answer: All of the above
- 10. On Ti-plasmid T-region or T-DNA is flanked by a direct repeat of
Options- A. 12 bp
- B. 20 bp
- C. 25 bp
- D. 30 bp Discuss
Correct Answer: 25 bp
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