Vectors Uses for Animal Cell Culture problems
- 1. Which of the following is/are commonly used promoter of S. cerevisiae?
Options- A. Galactokinase (GAL 1)
- B. Alcohol dehydrogenase II (ADH 2)
- C. Phosphoglycerate kinase (PGK)
- D. All of the above Discuss
Correct Answer: All of the above
- 2. Polyhedrin protein is
Options- A. non essential for baculo virus replication
- B. major component of protein matrix in which viruses are embedded
- C. both (a) and (b)
- D. essential for baculo virus replication Discuss
Correct Answer: both (a) and (b)
- 3. SV 40 vectors, in which late region replacements are done, require
Options- A. COS cell line
- B. SV 40 helper virus
- C. pBR 322 vector
- D. none of these Discuss
Correct Answer: SV 40 helper virus
- 4. Which of the following histone is not present in SV40 virus?
Options- A. H1
- B. H2A
- C. H3
- D. H4 Discuss
Correct Answer: H1
- 5. Retroviruses consist of
Options- A. Ds DNA genome
- B. Ss DNA genome
- C. RNA genome
- D. cDNA genome Discuss
Correct Answer: RNA genome
- 6. Autographa California nuclear polyhedrosis virus that is used as a vector for animal cells is an example of
Options- A. Vaccinia virus
- B. Baculo virus
- C. Papilloma virus
- D. Retrovirus Discuss
Correct Answer: Baculo virus
- 7. Lytic infection of SV 40 does not occur in
Options- A. permissive cells
- B. non permissive cells
- C. both (a) and (b)
- D. none of these Discuss
Correct Answer: non permissive cells
- 8. YAC vectors can be used to obtain artificial chromosomes because it carries
Options- A. centromere
- B. telomere
- C. autonomously replicating sequence
- D. all of these Discuss
Correct Answer: all of these
- 9. Which of the following are produced in high copy number?
Options- A. YEp
- B. YRp
- C. Both (a) and (b)
- D. YCp Discuss
Correct Answer: Both (a) and (b)
- 10. Maximum insert size in case of adenovirus vectors is
Options- A. 6-8 kb
- B. 4-6 kb
- C. 10-12 kb
- D. 20 kb Discuss
Correct Answer: 6-8 kb
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