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Media Required for Plant Cell Culture problems
1. Nitrogen in the plant cell culture media is provided by either ammonia or nitrate salt. In the media
Options
A. utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise
B. utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise
C. utilization of both ammonium and nitrate result in rise in pH
D. utilization of both ammonium and nitrate result in drop in pH
Show Answer
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Correct Answer: utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise
2. In plant cell culture media, auxins and cytokinins are used in the range of
Options
A. l-50?M
B. 50-100?M
C. 100-125?M
D. more than 125?M
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Correct Answer: l-50?M
3. Which is/are the disadvantage/(s) of using IAA in plant cell culture media?
Options
A. It is unstable in solution
B. Gets easily oxidized
C. Conjugated to inactive form by plant cells
D. All of the above
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Correct Answer: All of the above
4. What are the macronutrients used in plant cell culture medium?
Options
A. N, P, K, S, Na
B. N, P, K, Ca, Cl
C. N, P, K, S, Ca
D. N, P, Ca, Na, Cl
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Correct Answer: N, P, K, S, Ca
5. Which is/are the naturally occurring plant auxins?
Options
A. Indole acetic acid (IAA)
B. Naphthalenacetic acid (NAA)
C. 2,4-dichlorophenoxyacetic acid
D. All of the above
Show Answer
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Correct Answer: Indole acetic acid (IAA)
6. Which of the following growth regulator is added for short initiation during plant regeneration from callus?
Options
A. Auxins
B. Cytokinins
C. Gibberellins
D. Brassinosteroids
Show Answer
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Correct Answer: Cytokinins
7. Neutralized activated charcoal is occasionally added to young regenerating cultures to
Options
A. remove toxic phenolics produced by the stressed plant cell
B. help to remove plants growth regulators introduced at an earlier stage
C. both (a) and (b)
D. maintain the pH of the medium
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Correct Answer: both (a) and (b)
8. Which of the following is not a cytokinin?
Options
A. 2,4-dichlorophenoxyacetic acid
B. 6 benzylaminopurine
C. Zeatin
D. Kinetin
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Correct Answer: 2,4-dichlorophenoxyacetic acid
9. Which of the following growth regulator cause plant cells to grow?
Options
A. Auxins
B. Cytokinins
C. Gibberellins
D. Brassinosteroids
Show Answer
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Correct Answer: Auxins
10. Very high sugar concentration (40-100 g/l) have been used
Options
A. in specialized secondary metabolite production
B. to adjust the osmotic potential of the media in short term treatment for regeneration
C. Both (a) and (b)
D. none of these
Show Answer
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Correct Answer: Both (a) and (b)
First
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