Animal Cell Culture and Regulation problems
- 1. Excess CO2 suppress cell growth and productivity by
Options- A. inhibiting respiration
- B. altering intracellular pH by diffusing across cell membrane
- C. both (a) and (b)
- D. altering pH of the medium Discuss
Correct Answer: both (a) and (b)
- 2. Which of the following is correct?
Options- A. ECL can be established in suspension cultures whereas it is exceptional for primary cell lines (PCL)
- B. ECL and PCL can be established in suspension cultures
- C. PCL can be established in suspension cultures
- D. none of the above Discuss
Correct Answer: ECL can be established in suspension cultures whereas it is exceptional for primary cell lines (PCL)
- 3. The major problem associated with the isolation of free cells and cell aggregates from organs is that of
Options- A. releasing the cells from their supporting matrix
- B. inhibiting the cells from their supporting matrix
- C. disintegrating the cells from their supporting matrix
- D. none of the above Discuss
Correct Answer: releasing the cells from their supporting matrix
- 4. What are the main constituents of culture for animal cell growth?
Options- A. Glucose and Glutamine
- B. Growth factors
- C. Cytokines
- D. All of these Discuss
Correct Answer: Glucose and Glutamine
- 5. Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called
Options- A. established cell lines
- B. primary cell lines
- C. secondary cell lines
- D. propagated cell lines Discuss
Correct Answer: established cell lines
- 6. Range of optimum glutamine concentration present in the culture media is
Options- A. 1-2 mmol/litre
- B. 2-7 mmol/litre
- C. 7-15 mmol/litre
- D. 15 - 20 mmol/litre Discuss
Correct Answer: 2-7 mmol/litre
- 7. Which of the following is not a source of energy in active muscle cells?
Options- A. Creatine phosphate
- B. ATP
- C. Lactic acid
- D. Glucose Discuss
Correct Answer: Lactic acid
- 8. The human fibroplast is a classical example of
Options- A. stable primary cell lines
- B. established cell lines
- C. cell transformations
- D. none of these Discuss
Correct Answer: stable primary cell lines
- 9. When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of
Options- A. incomplete glutamine oxidation
- B. increase in specific lactate production from glucose
- C. both (a) and (b)
- D. accumulation of ammonia Discuss
Correct Answer: both (a) and (b)
- 10. During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture?
Options- A. Ethyl alcohol is being produced in excess
- B. The cells have too much oxygen
- C. Glycolysis is being inhibited
- D. The cells do not have enough oxygen Discuss
Correct Answer: The cells do not have enough oxygen
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