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Recombinant DNA problems


  • 1. Bacterial cells protect their own DNA from degradation by restriction endonucleases by

  • Options
  • A. methylating the DNA at the sites that the enzyme recognizes
  • B. deleting all recognition sites from the genome
  • C. not producing any restriction endonucleases
  • D. having anti restriction endonucleases
  • Discuss
  • 2. Agrobacterium tumefaciens is

  • Options
  • A. a disease in humans that causes loss of sight
  • B. a bacterium that can be used to introduce DNA into plants
  • C. a fungi that is used to produce antibiotics in large amounts
  • D. a disease in humans that causes loss of weight
  • Discuss
  • 3. Problems in obtaining large amounts of proteins encoded by recombinant genes can often be overcome by using

  • Options
  • A. BACS
  • B. expression vectors
  • C. YACS
  • D. all of these
  • Discuss
  • 4. A genomic library is

  • Options
  • A. a database where the sequence of an organism's genome is stored
  • B. a collection of many clones possessing different DNA fragments from the same organisms bound to vectors
  • C. a book that describes how to isolate DNA from a particular organism
  • D. a place where the information of the genetic organization of organisms are kept.
  • Discuss
  • 5. Which of the following is obtained using processed mRNA molecules as a template?

  • Options
  • A. rDNA
  • B. mDNA
  • C. cDNA
  • D. tDNA
  • Discuss
  • 6. Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?

  • Options
  • A. DNA polymerase
  • B. Restriction endonuclease
  • C. DNA ligase
  • D. DNA helicase
  • Discuss
  • 7. Vectors are

  • Options
  • A. molecules that degrade nucleic acids
  • B. molecules that help in replication
  • C. molecules that are able to covalently bond to and carry foreign DNA into cells
  • D. molecules that protect host cells from invasion by foreign DNA
  • Discuss
  • 8. Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are

  • Options
  • A. DNA ligase
  • B. helicases
  • C. restriction endonucleases
  • D. DNA gyrase
  • Discuss
  • 9. Electroporation is

  • Options
  • A. the process of separating charged molecules through a gel maintained in an electric field
  • B. the process of combining foreign DNA to an electrically charged vector molecule
  • C. the application of high voltage pulses
  • D. the process of multiplication of the cells
  • Discuss
  • 10. Which of the following is not commonly used as vector?

  • Options
  • A. Artificial chromosome
  • B. Cosmid
  • C. Fungi
  • D. Plasmid
  • Discuss

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