Recombinant DNA problems
- 1. Bacterial cells protect their own DNA from degradation by restriction endonucleases by
Options- A. methylating the DNA at the sites that the enzyme recognizes
- B. deleting all recognition sites from the genome
- C. not producing any restriction endonucleases
- D. having anti restriction endonucleases Discuss
Correct Answer: methylating the DNA at the sites that the enzyme recognizes
- 2. Agrobacterium tumefaciens is
Options- A. a disease in humans that causes loss of sight
- B. a bacterium that can be used to introduce DNA into plants
- C. a fungi that is used to produce antibiotics in large amounts
- D. a disease in humans that causes loss of weight Discuss
Correct Answer: a bacterium that can be used to introduce DNA into plants
- 3. Problems in obtaining large amounts of proteins encoded by recombinant genes can often be overcome by using
Options- A. BACS
- B. expression vectors
- C. YACS
- D. all of these Discuss
Correct Answer: expression vectors
- 4. A genomic library is
Options- A. a database where the sequence of an organism's genome is stored
- B. a collection of many clones possessing different DNA fragments from the same organisms bound to vectors
- C. a book that describes how to isolate DNA from a particular organism
- D. a place where the information of the genetic organization of organisms are kept. Discuss
Correct Answer: a collection of many clones possessing different DNA fragments from the same organisms bound to vectors
- 5. Which of the following is obtained using processed mRNA molecules as a template?
Options- A. rDNA
- B. mDNA
- C. cDNA
- D. tDNA Discuss
Correct Answer: cDNA
- 6. Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?
Options- A. DNA polymerase
- B. Restriction endonuclease
- C. DNA ligase
- D. DNA helicase Discuss
Correct Answer: DNA ligase
- 7. Vectors are
Options- A. molecules that degrade nucleic acids
- B. molecules that help in replication
- C. molecules that are able to covalently bond to and carry foreign DNA into cells
- D. molecules that protect host cells from invasion by foreign DNA Discuss
Correct Answer: molecules that are able to covalently bond to and carry foreign DNA into cells
- 8. Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are
Options- A. DNA ligase
- B. helicases
- C. restriction endonucleases
- D. DNA gyrase Discuss
Correct Answer: restriction endonucleases
- 9. Electroporation is
Options- A. the process of separating charged molecules through a gel maintained in an electric field
- B. the process of combining foreign DNA to an electrically charged vector molecule
- C. the application of high voltage pulses
- D. the process of multiplication of the cells Discuss
Correct Answer: the application of high voltage pulses
- 10. Which of the following is not commonly used as vector?
Options- A. Artificial chromosome
- B. Cosmid
- C. Fungi
- D. Plasmid Discuss
Correct Answer: Fungi
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