Plant Cell Culture problems
- 1. Cell fusion method includes the preparation of large number of
Options- A. plant cells stripped of their cell wall
- B. single plant cell stripped of their cell wall
- C. plant cells with cell wall
- D. cells from different species Discuss
Correct Answer: single plant cell stripped of their cell wall
- 2. Which of the following is considered as the disadvantage of conventional plant tissue culture for clonal propagation?
Options- A. Multiplication of sexually derived sterile hybrids
- B. Less multiplication of disease free plants
- C. Storage and transportation of propogates
- D. Both (b) and (c) Discuss
Correct Answer: Storage and transportation of propogates
- 3. Protoplasts can be produced from suspension cultures, callus tissues or intact tissues by enzymatic treatment with
Options- A. cellulotyic enzymes
- B. pectolytic enzymes
- C. both cellulotyic and pectolytic enzymes
- D. proteolytic enzymes Discuss
Correct Answer: both cellulotyic and pectolytic enzymes
- 4. The ability of the component cells of callus to form a whole plant is known as
Options- A. redifferentiation
- B. dedifferentiation
- C. either (a) or (b)
- D. none of these Discuss
Correct Answer: redifferentiation
- 5. When plated only in nutrient medium, how much time is required for the protoplast to synthesize new cell wall?
Options- A. 2-5 days
- B. 5-10 days
- C. 10-15 days
- D. 15-17 days Discuss
Correct Answer: 5-10 days
- 6. Which method of plant propagation involves the use of girdling?
Options- A. Grafting
- B. Cuttings
- C. Layering
- D. Micropropagation Discuss
Correct Answer: Layering
- 7. Subculturing is similar to propagation by cuttings because
Options- A. it separates multiple microshoots and places them in a medium
- B. it uses scions to produce new microshoots
- C. they both use in vitro growing conditions
- D. all of the above Discuss
Correct Answer: it separates multiple microshoots and places them in a medium
- 8. What is/are the benefit(s) of micropropagation or clonal propagation?
Options- A. Rapid multiplication of superior clones
- B. Multiplication of disease free plants
- C. Multiplication of sexually derived sterile hybrids
- D. All of the above Discuss
Correct Answer: All of the above
- 9. Agrobacterium based gene transfer is efficient
Options- A. only with dicots
- B. only with monocots
- C. with both monocots and dicots
- D. with majority monocots and few dicots Discuss
Correct Answer: only with dicots
- 10. In a callus culture
Options- A. increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation
- B. increasing level of auxin to a callus induces shoot formation and increasing level of cytokinin promote root formation
- C. auxins and cytokinins are not required
- D. only auxin is required for root and shoot formation Discuss
Correct Answer: increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation
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