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  • Question
  • What is the normal role of restriction endonucleases in bacterial cells?


  • Options
  • A. To degrade the bacterial chromosome into small pieces during replication
  • B. To degrade invading phage DNA
  • C. To produce RNA primers for replication
  • D. All of the above

  • Correct Answer
  • To degrade invading phage DNA 


  • Recombinant DNA problems


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    • 1. The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of

    • Options
    • A. genetic engineering
    • B. population genetics
    • C. microbiology
    • D. protein engineering
    • Discuss
    • 2. Which of the following vector can maintain the largest fragment of foreign DNA?

    • Options
    • A. YAC
    • B. Cosmid
    • C. Plasmid
    • D. Phage
    • Discuss
    • 3. In genetic engineering, a chimera is

    • Options
    • A. an enzyme that links DNA molecules
    • B. a plasmid that contains foreign DNA
    • C. a virus that infects bacteria
    • D. a fungi
    • Discuss
    • 4. The Southern blotting technique depends on

    • Options
    • A. similarities between the sequences of probe DNA and experimental DNA
    • B. similarities between the sequences of probe RNA and experimental RNA
    • C. similarities between the sequences of probe protein and experimental protein
    • D. the molecular mass of proteins
    • Discuss
    • 5. A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as

    • Options
    • A. southern blotting
    • B. northern blotting
    • C. polymerase chain reaction
    • D. DNA replication
    • Discuss
    • 6. The advantage of using DNA polymerases from thermophilic organisms in PCR is that

    • Options
    • A. the DNA polymerases of these bacteria are much faster than those from other organisms
    • B. the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands
    • C. the DNA polymerases of these bacteria never make mistakes while replicating DNA
    • D. all of the above
    • Discuss
    • 7. For gene probes to be useful they must

    • Options
    • A. be large enough to contain gene-specific sequences
    • B. be labeled in some manner to allow detection
    • C. both (a) and (b)
    • D. none of the above
    • Discuss
    • 8. Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as

    • Options
    • A. photoreactivation
    • B. gel electrophoresis
    • C. autoradiography
    • D. blotting
    • Discuss
    • 9. The piece of equipment, that introduces DNA into cells via DNA-coated microprojectiles is known as

    • Options
    • A. laser
    • B. DNA probe
    • C. gene gun
    • D. inoculating needle
    • Discuss
    • 10. Which type of restriction endonuclease cuts the DNA within the recognition site?

    • Options
    • A. Type I
    • B. Type II
    • C. Type III
    • D. All of the these
    • Discuss


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