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Home Biotechnology Recombinant DNA Comments

  • Question
  • A short molecule containing 2-20 nucleotide is


  • Options
  • A. plasmid
  • B. vector
  • C. oligonucleotide
  • D. mononucleotide

  • Correct Answer
  • oligonucleotide 


  • Recombinant DNA problems


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    • 1. An animal, that has gained new genetic information from the acquisition of foreign DNA, is considered as

    • Options
    • A. a chimera
    • B. a transgenic animal
    • C. a vector
    • D. an enzyme that links DNA molecules
    • Discuss
    • 2. Which of the following is not commonly used as vector?

    • Options
    • A. Artificial chromosome
    • B. Cosmid
    • C. Fungi
    • D. Plasmid
    • Discuss
    • 3. Electroporation is

    • Options
    • A. the process of separating charged molecules through a gel maintained in an electric field
    • B. the process of combining foreign DNA to an electrically charged vector molecule
    • C. the application of high voltage pulses
    • D. the process of multiplication of the cells
    • Discuss
    • 4. Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are

    • Options
    • A. DNA ligase
    • B. helicases
    • C. restriction endonucleases
    • D. DNA gyrase
    • Discuss
    • 5. Vectors are

    • Options
    • A. molecules that degrade nucleic acids
    • B. molecules that help in replication
    • C. molecules that are able to covalently bond to and carry foreign DNA into cells
    • D. molecules that protect host cells from invasion by foreign DNA
    • Discuss
    • 6. A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as

    • Options
    • A. southern blotting
    • B. northern blotting
    • C. polymerase chain reaction
    • D. DNA replication
    • Discuss
    • 7. The Southern blotting technique depends on

    • Options
    • A. similarities between the sequences of probe DNA and experimental DNA
    • B. similarities between the sequences of probe RNA and experimental RNA
    • C. similarities between the sequences of probe protein and experimental protein
    • D. the molecular mass of proteins
    • Discuss
    • 8. In genetic engineering, a chimera is

    • Options
    • A. an enzyme that links DNA molecules
    • B. a plasmid that contains foreign DNA
    • C. a virus that infects bacteria
    • D. a fungi
    • Discuss
    • 9. Which of the following vector can maintain the largest fragment of foreign DNA?

    • Options
    • A. YAC
    • B. Cosmid
    • C. Plasmid
    • D. Phage
    • Discuss
    • 10. The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of

    • Options
    • A. genetic engineering
    • B. population genetics
    • C. microbiology
    • D. protein engineering
    • Discuss


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