Gene Transfer in Plants problems
- 1. Which of the following is not true about the direct repeats flanking T-DNA?
Options- A. They are conserved between nopaline and octopine Ti-plasmids
- B. These repeats are transferred intact to the plant genome
- C. These are important in integration mechanism
- D. all of the above Discuss
Correct Answer: These repeats are transferred intact to the plant genome
- 2. The direct repeats flanking the T-DNA of Agrobacterium tume-faciens are known as
Options- A. cos site
- B. flanking sequences
- C. border sequences
- D. transfer sequences Discuss
Correct Answer: border sequences
- 3. Plant transformation vectors based on Agrobacterium can generally be divided into
Options- A. two vectors
- B. four vectors
- C. six vectors
- D. eight vectors Discuss
Correct Answer: two vectors
- 4. The size of the virulent plasmid of Agrobacterium tumefaciens is
Options- A. 40-80 kb
- B. 80-120 kb
- C. 140-235 kb
- D. >235 kb Discuss
Correct Answer: 140-235 kb
- 5. Advantage of microprojectile method over microinjection method for gene transfer in plants include
Options- A. intact cells are used
- B. method is universal in its application irrespective of all shape, size, type and presence or absence of cell wall
- C. gene can be transferred to many cells simultaneously
- D. all of the above Discuss
Correct Answer: all of the above
- 6. Opines are
Options- A. amino acid derivatives found in tumor tissues
- B. amino acid derivatives found in normal tissues
- C. amino acid derivatives found in both normal as well as tumor tissues
- D. none of the above Discuss
Correct Answer: amino acid derivatives found in tumor tissues
- 7. Agrobacterium tumefaciens is a
Options- A. gram (+) bacteria
- B. gram (-) bacteria
- C. a fungi
- D. a yeast Discuss
Correct Answer: gram (-) bacteria
- 8. Co-integrating transformation vectors must include a region of homology in
Options- A. the vector plasmid
- B. the Ti-plasmid
- C. between vector plasmid and Ti-plasmid
- D. none of these Discuss
Correct Answer: between vector plasmid and Ti-plasmid
- 9. Microprojectile method of gene transfer in plants involves delivery of DNA
Options- A. with the help of micromanipulator
- B. with the help of bolistics
- C. with the help of needles
- D. any of the above Discuss
Correct Answer: with the help of bolistics
- 10. Which of the following is not true about the helper plasmids?
Options- A. These can replicate in Agrobacterium
- B. These help in the mediating conjugation of intermediate vectors
- C. These can't replicate in Agrobacterium
- D. All of the above Discuss
Correct Answer: These can replicate in Agrobacterium
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