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Gene Transfer in Plants problems


  • 1. Which of the following genes are constitutively expressed and control the plant induced activation of other vir genes?

  • Options
  • A. vir A and vir G
  • B. vir C and vir D
  • C. vir B and vir E
  • D. vir A and vir B
  • Discuss
  • 2. Opine synthesis is the property

  • Options
  • A. conferred to plant cells when it transformed by Agrobacterium tumefaciens
  • B. determined by the bacteria Agrobacterium tumefaciens
  • C. both (a) and (b)
  • D. of normal plant cells
  • Discuss
  • 3. In response to the activating signal molecule, an endonuclease is produced that causes nicks in the T-DNA. It is encoded by

  • Options
  • A. vir A
  • B. vir B
  • C. vir C
  • D. vir D
  • Discuss
  • 4. The transfer of intermediate vectors into Agrobacterium are brought about by

  • Options
  • A. transformation
  • B. biparental mating
  • C. triparental mating
  • D. transduction
  • Discuss
  • 5. T-DNA transfer and processing into plant genome requires products of which of the following genes?

  • Options
  • A. vir A,B
  • B. vir G,C
  • C. vir D,E
  • D. All of these
  • Discuss
  • 6. Direct DNA uptake by protoplasts can be stimulated by

  • Options
  • A. polyethylene glycol (PEG)
  • B. decanal
  • C. luciferin
  • D. all of these
  • Discuss
  • 7. Liposomes mediated gene transfer in plants involves

  • Options
  • A. plasmid DNA enclosed in a lipid bag
  • B. fusion of liposomes with protoplast
  • C. use of polyethylene glycol (PEG)
  • D. all of the above
  • Discuss
  • 8. The left segment of octopine T-DNA (TL) is necessary for

  • Options
  • A. enzymes for agropine biosynthesis
  • B. tumour formation
  • C. conjugative transfer
  • D. all of these
  • Discuss
  • 9. Integrated nopaline T-DNA occurs as

  • Options
  • A. single segment
  • B. two segments
  • C. three segments
  • D. four segments
  • Discuss
  • 10. In the liposome mediated gene transfer in plants, nucleic acids are

  • Options
  • A. protected from nuclease digestion
  • B. stable in liposomes
  • C. both (a) and (b)
  • D. not stable in liposomes
  • Discuss

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