Gene Transfer in Plants problems
- 1. Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?
Options- A. pRK 2013
- B. pRN 3
- C. either (a) or (b)
- D. none of these Discuss
Correct Answer: either (a) or (b)
- 2. Microinjection involves
Options- A. injection of large amount of DNA
- B. injection of DNA into bigger cells
- C. injection with needle having diameter greater than cell diameter
- D. all of the above Discuss
Correct Answer: injection with needle having diameter greater than cell diameter
- 3. Which of the following is true about T DNA?
Options- A. Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA
- B. Integration of T DNA occurs only at one specific sites in the plant nuclear DNA
- C. Integration of T DNA occurs at two specific sites in the plant nuclear DNA
- D. Integration of T DNA occurs at one site that may be random in the plant nuclear DNA Discuss
Correct Answer: Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA
- 4. Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain
Options- A. one set of sequence necessary for gene transfer
- B. two sets of sequence necessary for gene transfer
- C. three sets of sequence necessary for gene transfer
- D. four sets of sequence necessary for gene transfer Discuss
Correct Answer: two sets of sequence necessary for gene transfer
- 5. Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into
Options- A. pBR 322 based plasmid vector
- B. pRK 2013
- C. pRN 3
- D. all of these Discuss
Correct Answer: pBR 322 based plasmid vector
- 6. Which of the following plant signal molecules regulate the expression of vir B, C, D and E in case of tobacco?
Options- A. Acetosyringone
- B. ?-hydroxy syringone
- C. Both (a) and (b)
- D. None of these Discuss
Correct Answer: Both (a) and (b)
- 7. Which of the following is not true for microinjection technique that involves transfer of DNA into protoplast?
Options- A. It is carried out with the help of micromanipulator
- B. The recipient cells are immobilized on artificial support or artificially bound to substarate
- C. It employs needle with diameter greater than cell diameter
- D. all of the above Discuss
Correct Answer: It employs needle with diameter greater than cell diameter
- 8. In a plant tumour cell
Options- A. complete Ti plasmid is incorporated in plant nuclear DNA
- B. different parts of the Ti-plasmid are incorporated
- C. only a small specific segment of callus T DNA is incorporated
- D. may vary from plant to plant Discuss
Correct Answer: only a small specific segment of callus T DNA is incorporated
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