Animal Cell Culture and Regulation problems
- 1. At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via
Options- A. oxidative pathway
- B. anaerobic pathway
- C. both (a) and (b)
- D. none of these Discuss
Correct Answer: oxidative pathway
- 2. Toxicity due to accumulation of ammonia can be overcome
Options- A. by substituting glutamine by glutamate
- B. by controlled addition of glutamine at low level
- C. by removal of ammonia or ammonium from culture medium
- D. all of the above Discuss
Correct Answer: all of the above
- 3. Which of the following is incorrect?
Options- A. Established cell lines (ECL) have short doubling time
- B. ECL are invariably aneuploid
- C. ECL grow in higher density
- D. ECL do not show much evidence of spatial orientation Discuss
Correct Answer: ECL grow in higher density
- 4. The technique of organ culture may be divided on the basis of employing
Options- A. solid medium
- B. liquid medium
- C. both (a) and (b)
- D. semi-solid medium Discuss
Correct Answer: both (a) and (b)
- 5. According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of
Options- A. 6 amino acids
- B. 8 amino acids
- C. 10 amino acids
- D. 12 amino acids Discuss
Correct Answer: 12 amino acids
- 6. What is the concentration of CO2 required for culturing animal cells?
Options- A. 2-5%
- B. 1-10%
- C. 10-15%
- D. 15-20% Discuss
Correct Answer: 1-10%
- 7. Accumulation of lactate leads to
Options- A. increase in pH
- B. no change in pH
- C. reduction in the pH of culture hence loss of cell viability
- D. no loss of cell viability Discuss
Correct Answer: reduction in the pH of culture hence loss of cell viability
- 8. Which of the following is the technique used for the embryo culture?
Options- A. Organ cultures on plasma clots
- B. Organ cultures on agar
- C. Whole embryo cultures
- D. All of these Discuss
Correct Answer: All of these
- 9. To prevent the accumulation of lactate
Options- A. low glutamine concentration is required
- B. high glutamine concentration is required
- C. low glucose concentration is required
- D. high glucose concentration is required Discuss
Correct Answer: low glucose concentration is required
- 10. How does CO2 help in the cell metabolism during cell culture?
Options- A. It participates in the de novo synthesis of purines and pyrimidines
- B. Helps in the cells respiration
- C. For monitoring pH of the culture
- D. All of the above Discuss
Correct Answer: It participates in the de novo synthesis of purines and pyrimidines
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