Animal Cell Culture and Regulation problems
- 1. What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?
Options- A. They act as growth promoters
- B. They act as growth inhibitors
- C. Have no effect on cells
- D. Lactate helps in the growth while ammonium inhibits the growth Discuss
Correct Answer: Lactate helps in the growth while ammonium inhibits the growth
- 2. Higher dissolved oxygen concentration in the culture media are toxic and lead to
Options- A. DNA degradation
- B. lipid peroxidation
- C. metabolism of nutrients in culture media at a rate greater than that required for consumption
- D. all of the above Discuss
Correct Answer: all of the above
- 3. pH of culture medium is initially controlled by
Options- A. presence of CO2
- B. presence of bicarbonate buffer
- C. addition of bases
- D. none of these Discuss
Correct Answer: presence of bicarbonate buffer
- 4. Disaggregating of cells can be achieved by
Options- A. physical disruption
- B. enzymatic digestion
- C. treating with chelating agents
- D. all of the above Discuss
Correct Answer: all of the above
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