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Home Biotechnology Animal Cell Culture and Regulation Comments

  • Question
  • The major problem associated with the isolation of free cells and cell aggregates from organs is that of


  • Options
  • A. releasing the cells from their supporting matrix
  • B. inhibiting the cells from their supporting matrix
  • C. disintegrating the cells from their supporting matrix
  • D. none of the above

  • Correct Answer
  • releasing the cells from their supporting matrix 


  • Animal Cell Culture and Regulation problems


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    • 1. Which of the following is correct?

    • Options
    • A. ECL can be established in suspension cultures whereas it is exceptional for primary cell lines (PCL)
    • B. ECL and PCL can be established in suspension cultures
    • C. PCL can be established in suspension cultures
    • D. none of the above
    • Discuss
    • 2. Excess CO2 suppress cell growth and productivity by

    • Options
    • A. inhibiting respiration
    • B. altering intracellular pH by diffusing across cell membrane
    • C. both (a) and (b)
    • D. altering pH of the medium
    • Discuss
    • 3. Klinefelter syndrome is a manifestation of which chromosome aneuploidy?

    • Options
    • A. XXX
    • B. XYY
    • C. XXY
    • D. None of these
    • Discuss
    • 4. Which of the following sets belongs to a distinct class or category?

    • Options
    • A. Ribose, sucrose, glucose, fructose
    • B. Sterols, waxes, fatty acids, amino acids.
    • C. Oils, sterols, waxes, fats
    • D. Nucleotide, nucleoside, nucleus, DNA
    • Discuss
    • 5. If the RBC of human were kept in 0.9% NaCl solution, what sort of change will occur to RBC?

    • Options
    • A. Cell will Burst
    • B. Cell will shrink
    • C. No change to RBC
    • D. RBC will destroy
    • Discuss
    • 6. What are the main constituents of culture for animal cell growth?

    • Options
    • A. Glucose and Glutamine
    • B. Growth factors
    • C. Cytokines
    • D. All of these
    • Discuss
    • 7. Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

    • Options
    • A. established cell lines
    • B. primary cell lines
    • C. secondary cell lines
    • D. propagated cell lines
    • Discuss
    • 8. Range of optimum glutamine concentration present in the culture media is

    • Options
    • A. 1-2 mmol/litre
    • B. 2-7 mmol/litre
    • C. 7-15 mmol/litre
    • D. 15 - 20 mmol/litre
    • Discuss
    • 9. Which of the following is not a source of energy in active muscle cells?

    • Options
    • A. Creatine phosphate
    • B. ATP
    • C. Lactic acid
    • D. Glucose
    • Discuss
    • 10. The human fibroplast is a classical example of

    • Options
    • A. stable primary cell lines
    • B. established cell lines
    • C. cell transformations
    • D. none of these
    • Discuss


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