Vectors Uses for Animal Cell Culture problems
- 1. Which one of the following is the most stable vector?
Options- A. YEp
- B. YRp
- C. YAC
- D. YIp Discuss
Correct Answer: YAC
- 2. SV 40 vectors, in which early region replacements are carried out, require
Options- A. COS cell line
- B. SV 40 helper virus
- C. pBR 322 vector
- D. none of these Discuss
Correct Answer: COS cell line
- 3. Which one of the following is true about Yeast Integrative plasmids (YIp) vectors?
Options- A. Allows transformation by using crossing over
- B. Has a origin of replication and rep genes
- C. Carries autonomously replicating sequence
- D. Carries centromere and telomere sequences Discuss
Correct Answer: Allows transformation by using crossing over
- 4. Which one of the following is true about Yeast Centromeric plasmids (YCp) vectors?
Options- A. Contains centromeric sequences
- B. Segregates during meiosis and mitosis
- C. Functions as true chromosome
- D. All of the above Discuss
Correct Answer: All of the above
- 5. Which of the following can be used for reducing the size of gene inserts containing introns?
Options- A. Vaccinia virus
- B. Baculo virus
- C. Retro virus
- D. none of these Discuss
Correct Answer: Retro virus
- 6. Autonomously replicating sequence is not present in
Options- A. YIp
- B. YRp
- C. YCp
- D. YAC Discuss
Correct Answer: YIp
- 7. Maximum amount of foreign DNA introduced into SV 40 viruses is
Options- A. 10 kb
- B. 5 kb
- C. 2.5 kb
- D. 1 kb Discuss
Correct Answer: 2.5 kb
- 8. Which of the following promoters is repressed in presence of glucose?
Options- A. Galactokinase (GAL 1)
- B. Acid phosphatase (PHOS)
- C. Both (a) and (b)
- D. Phosphoglycerate kinase (PGK) Discuss
Correct Answer: Galactokinase (GAL 1)
- 9. In case of Baculoviruses, the foreign gene
Options- A. is inserted downstream of polyhedrin promoter
- B. is inserted downstream of viral origin of replication
- C. is inserted between the polyhedrin promoter and downstream sequences
- D. is inserted randomly in viral genome Discuss
Correct Answer: is inserted between the polyhedrin promoter and downstream sequences
- 10. Bovine papilloma virus has a circular double stranded DNA genome of about
Options- A. 2.5 kb
- B. 5.2 kb
- C. 7.9 kb
- D. 9.5 kb Discuss
Correct Answer: 7.9 kb
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