Vectors Uses for Animal Cell Culture problems
- 1. Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA
Options- A. episomally only
- B. integrated only
- C. either episomally or integrated at high copy number
- D. either episomally or integrated at low copy number Discuss
Correct Answer: either episomally or integrated at high copy number
- 2. Recombinant SV40 viruses introduce the foreign DNA into cells
Options- A. with the DNA mediated transfection
- B. without the DNA mediated transfection
- C. with the RNA mediated transfection
- D. without the RNA mediated transfection Discuss
Correct Answer: without the DNA mediated transfection
- 3. Which of the following(s) is/are the most commonly used markers in yeast?
Options- A. His 3
- B. Ura 3
- C. Trp 1
- D. All of these Discuss
Correct Answer: All of these
- 4. The size of the genome of SV 40 is
Options- A. 7.2 kb
- B. 69 kb
- C. 5.2 kb
- D. 110 kb Discuss
Correct Answer: 5.2 kb
- 5. Retroviruses have advantage for being used as vector for animal cells because
Options- A. they cover a wide host range
- B. infection does not lead to cell death, infected cells produce virus over an indefinite period
- C. viral gene expression is driven by strong promoters
- D. all of the above Discuss
Correct Answer: all of the above
- 6. Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?
Options- A. Allows transformation by using crossing over
- B. Has a origin of replication and rep genes
- C. Carries autonomously replicating sequence
- D. Carries centromere and telomere sequences Discuss
Correct Answer: Has a origin of replication and rep genes
- 7. Yeast Replicating plasmids (YRp) carry
Options- A. centromere
- B. telomere
- C. autonomously replicating sequence
- D. both (a) and (c) Discuss
Correct Answer: autonomously replicating sequence
- 8. Recombinant adeno virus can be created by deleting E1A/E 1B genes that are
Options- A. transcriptional regulators and are essential for viral replication
- B. not necessary for viral replication
- C. coding for virus coat protein
- D. important for packaging of viral DNA into viral head Discuss
Correct Answer: transcriptional regulators and are essential for viral replication
- 9. Cos cell line is
Options- A. cohesive end site of phage ?
- B. derivative of permissive CV-1 monkey cell line
- C. cohesive initial site of phage ?
- D. derivative of non-permissive CV-1 monkey cell line Discuss
Correct Answer: derivative of permissive CV-1 monkey cell line
- 10. Yeast episomal plasmids (YEp) were constructed using
Options- A. 2?m yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9
- B. 2?m yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322
- C. 12?m yeast plasmid and E coli vector pMB 9
- D. 12?m yeast plasmid and E coli vector pBR 322 Discuss
Correct Answer: 2?m yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9
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