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Home Biotechnology Recombinant DNA Comments

  • Question
  • Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are


  • Options
  • A. DNA ligase
  • B. helicases
  • C. restriction endonucleases
  • D. DNA gyrase

  • Correct Answer
  • restriction endonucleases 


  • Recombinant DNA problems


    Search Results


    • 1. Vectors are

    • Options
    • A. molecules that degrade nucleic acids
    • B. molecules that help in replication
    • C. molecules that are able to covalently bond to and carry foreign DNA into cells
    • D. molecules that protect host cells from invasion by foreign DNA
    • Discuss
    • 2. Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?

    • Options
    • A. DNA polymerase
    • B. Restriction endonuclease
    • C. DNA ligase
    • D. DNA helicase
    • Discuss
    • 3. Which of the following is obtained using processed mRNA molecules as a template?

    • Options
    • A. rDNA
    • B. mDNA
    • C. cDNA
    • D. tDNA
    • Discuss
    • 4. A genomic library is

    • Options
    • A. a database where the sequence of an organism's genome is stored
    • B. a collection of many clones possessing different DNA fragments from the same organisms bound to vectors
    • C. a book that describes how to isolate DNA from a particular organism
    • D. a place where the information of the genetic organization of organisms are kept.
    • Discuss
    • 5. Problems in obtaining large amounts of proteins encoded by recombinant genes can often be overcome by using

    • Options
    • A. BACS
    • B. expression vectors
    • C. YACS
    • D. all of these
    • Discuss
    • 6. Electroporation is

    • Options
    • A. the process of separating charged molecules through a gel maintained in an electric field
    • B. the process of combining foreign DNA to an electrically charged vector molecule
    • C. the application of high voltage pulses
    • D. the process of multiplication of the cells
    • Discuss
    • 7. Which of the following is not commonly used as vector?

    • Options
    • A. Artificial chromosome
    • B. Cosmid
    • C. Fungi
    • D. Plasmid
    • Discuss
    • 8. An animal, that has gained new genetic information from the acquisition of foreign DNA, is considered as

    • Options
    • A. a chimera
    • B. a transgenic animal
    • C. a vector
    • D. an enzyme that links DNA molecules
    • Discuss
    • 9. A short molecule containing 2-20 nucleotide is

    • Options
    • A. plasmid
    • B. vector
    • C. oligonucleotide
    • D. mononucleotide
    • Discuss
    • 10. A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as

    • Options
    • A. southern blotting
    • B. northern blotting
    • C. polymerase chain reaction
    • D. DNA replication
    • Discuss


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