Cloning Vectors problems
- 1. Plasmids which are maintained as limited number of copies per cell are known as
Options- A. stringent plasmids
- B. relaxed plasmids
- C. cryptic plasmids
- D. all of these Discuss
Correct Answer: stringent plasmids
- 2. Conjugative plasmids
Options- A. exhibit antibiotic resistance
- B. do not exhibit antibiotic resistance
- C. carry transfer genes called the tra genes
- D. do not carry transfer genes Discuss
Correct Answer: carry transfer genes called the tra genes
- 3. Plasmids which are maintained as multiple copy number per cell are known as
Options- A. stringent plasmids
- B. relaxed plasmids
- C. cryptic plasmids
- D. none of these Discuss
Correct Answer: relaxed plasmids
- 4. Maximum size of foreign DNA that can be inserted into a replacement vector is
Options- A. 25-30 kb
- B. 18-20 kb
- C. 20-25 kb
- D. 40-50 kb Discuss
Correct Answer: 20-25 kb
- 5. Select the wrong statement about plasmids?
Options- A. It is extrachromosomal
- B. It is double stranded
- C. Its replication depends upon host cell
- D. It is closed and circular DNA Discuss
Correct Answer: Its replication depends upon host cell
- 6. Charon 34 and Charon 35 can clone DNA upto
Options- A. 1-2 kb
- B. 6-7 kb
- C. 9-20 kb
- D. 30-35 kb Discuss
Correct Answer: 9-20 kb
- 7. Cosmid vectors are
Options- A. plasmids that contain fragment of ? DNA including the cos site
- B. phages that lack cos site
- C. plasmids that have no selection marker
- D. cryptic plasmids Discuss
Correct Answer: plasmids that contain fragment of ? DNA including the cos site
- 8. Single stranded vectors are useful
Options- A. for sequencing of cloned DNA
- B. for oligonucleotide directed mutagenesis
- C. for probe preparation
- D. all of the above Discuss
Correct Answer: all of the above
- 9. Stuffer is
Options- A. the right arm of the vector DNA
- B. the left arm of the vector DNA
- C. central fragment of the vector DNA
- D. none of the above Discuss
Correct Answer: central fragment of the vector DNA
- 10. Phagemid consist of
Options- A. plasmid vector carrying ? phage's cos site
- B. plasmid vector carrying ? attachment (? att) site
- C. plasmid vector carrying origin of replication of ? phage only
- D. plasmid vector carrying origin of replication of plasmid only Discuss
Correct Answer: plasmid vector carrying ? attachment (? att) site
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