Cloning Vectors problems
- 1. Cosmids lack
Options- A. genes coding for viral proteins
- B. origin of replication
- C. marker genes coding for replication
- D. cleavage site for the insertion of foreign DNA Discuss
Correct Answer: genes coding for viral proteins
- 2. A plasmid can be considered as a suitable cloning vector if
Options- A. it can be readily isolated from the cells
- B. it possesses a single restriction site for one or more restriction enzymes
- C. insertion of foreign DNA does not alter its replication properties
- D. All of the above Discuss
Correct Answer: All of the above
- 3. Which of the following is not true about phagemid?
Options- A. Contain functional origin of replication of the plasmid and ? phage
- B. May be propagated as a plasmid or as phage in appropriate strain
- C. Contain ? att site
- D. Can only be propagated as phage Discuss
Correct Answer: Can only be propagated as phage
- 4. Size of the DNA that can be packaged into a ? phage is
Options- A. 50 kb
- B. 35-53 kb
- C. 40-50 kb
- D. any size Discuss
Correct Answer: 35-53 kb
- 5. Inserted DNA in ? gt 11 can be expressed as
Options- A. ?-galactosidase fused protein
- B. free protein in the cytoplasm
- C. free protein that is secreted out
- D. all of the above Discuss
Correct Answer: ?-galactosidase fused protein
- 6. Cryptic plasmids
Options- A. do not exhibit any phenotypic trait
- B. exhibit many phenotypic traits
- C. exhibit one phenotypic traits
- D. exhibit antibiotic resistance Discuss
Correct Answer: do not exhibit any phenotypic trait
- 7. Charon vectors are different from EMBL vectors because
Options- A. they have more extensive range of restriction targets with in their polylinkers.
- B. physical separation of lambda arm from central fragment is required
- C. both (a) and (b)
- D. physical separation of lambda arm from central fragment is not required Discuss
Correct Answer: both (a) and (b)
- 8. EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to
Options- A. 6-7 kb
- B. 15-25 kb
- C. 40-44 kb
- D. 1-2 kb Discuss
Correct Answer: 15-25 kb
- 9. Cos site of the cosmids
Options- A. consists of 12 bases
- B. helps whole genome in circularization and ligation
- C. both (a) and (b)
- D. contains cleavage site Discuss
Correct Answer: both (a) and (b)
- 10. Maximum size of foreign DNA that can be inserted into an insertion vector is
Options- A. 35 kb
- B. 18 kb
- C. 50 kb
- D. 27 kb Discuss
Correct Answer: 18 kb
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