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Cell Cultures and Characteristics problems


  • 1. Which of the following techniques may be performed quantitatively to determine the number of bacteria of a particular type?

  • Options
  • A. Pour plate
  • B. Spread plate
  • C. Both (a) and (b)
  • D. Streak plate technique
  • Discuss
  • 2. In the process of freeze drying, a dense cell suspension is placed in small vials and is frozen at

  • Options
  • A. -60 to -78°C
  • B. -20 to -30°C
  • C. -30 to -48°C
  • D. -48 to -58°C
  • Discuss
  • 3. In the pour plate method, the mixed culture is diluted directly in tubes of

  • Options
  • A. liquid agar medium
  • B. sterile liquid usually water
  • C. both (a) and (b)
  • D. none of these
  • Discuss
  • 4. For the selection of endospore-forming bacteria, a mixed culture can be heated at

  • Options
  • A. 80°C for 10 minutes
  • B. 70°C for 10 minutes
  • C. 60°C for 10 minutes
  • D. 90°C for 10 minutes
  • Discuss
  • 5. To select cholera-causing bacterium, Vibrio cholerae, the pH of the medium is maintained at a pH of

  • Options
  • A. 8.5
  • B. 8.90
  • C. 6.79
  • D. 3.5
  • Discuss
  • 6. Important example(s) of enriched medium/media is/ are

  • Options
  • A. Loeffler's serum slope
  • B. Bordet-gengou medium
  • C. Blood agar
  • D. All of these
  • Discuss
  • 7. Intestinal bacteria can grow in the presence of __________ whereas nonintestinal bacteria are usually inhibited.

  • Options
  • A. bile salts
  • B. low concentration of various dyes
  • C. sugars
  • D. low levels of nitrogen
  • Discuss
  • 8. Agar is used for solidifying culture media because

  • Options
  • A. it does not affect by the growth of bacteria
  • B. it does not add to the nutritive properties of the medium
  • C. the melting and solidifying points of agar solution are not the same
  • D. all of the above
  • Discuss
  • 9. The medium which allows the growth of more than one microorganisms of interest but with morphologically distinguishable colonies is known as

  • Options
  • A. selective medium
  • B. enrichment medium
  • C. differential medium
  • D. none of these
  • Discuss
  • 10. Cell counting can be carried out by

  • Options
  • A. direct microscopic count using petroffhansser counting chamber
  • B. plate counting
  • C. membrane filter count
  • D. all of the above
  • Discuss

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