DNA Replication problems
- 1. Proofreading activity to maintain the fidelity of DNA synthesis
Options- A. occurs after the synthesis has been completed
- B. is a function of the 3'-5' exonuclease activity of the DNA polymerases
- C. requires the presence of an enzyme separate from the DNA polymerases
- D. occurs in prokaryotes but not eukaryotes Discuss
Correct Answer: is a function of the 3'-5' exonuclease activity of the DNA polymerases
- 2. Which of the following enzymes unwind short stretches of DNA helix immediately ahead of a replication fork?
Options- A. DNA polymerases
- B. Helicases
- C. Single-stranded binding proteins
- D. Topoisomerases Discuss
Correct Answer: Helicases
- 3. The replication of chromosomes by eukaryotes occurs in a relatively short period of time because
Options- A. the eukaryotes have more amount of DNA for replication
- B. the eukaryotic replication machinery is 1000 times faster than the prokaryotes
- C. each chromosome contains multiple replicons
- D. eukaryotic DNA is always single stranded Discuss
Correct Answer: each chromosome contains multiple replicons
- 4. During which of the following process a new copy of a DNA molecule is precisely synthesized?
Options- A. Trasformation
- B. Transcription
- C. Translation
- D. Replication Discuss
Correct Answer: Replication
- 5. The enzyme that catalyzes the synthesis of DNA is called
Options- A. DNA polymerase
- B. DNA gyrase
- C. DNA ligase
- D. helicase Discuss
Correct Answer: DNA polymerase
- 6. During DNA replication in prokaryotes, synthesis begins on the circular chromosome
Options- A. always at the same place
- B. at any stretch of DNA that is high in AT pairs
- C. randomly on the chromosome
- D. at the promoter Discuss
Correct Answer: always at the same place
- 7. Which of the following possesses both 5'-3' and 3'-5' exonuclease activity?
Options- A. Kornberg enzyme
- B. DNA polymerase III
- C. Taq DNA polymerase
- D. None of these Discuss
Correct Answer: Kornberg enzyme
- 8. DNA replication rates in prokaryotes are approximately of the order of
Options- A. 10 bases per second
- B. 1,00 bases per second
- C. 1,000 bases per second
- D. 10,000 bases per second Discuss
Correct Answer: 1,000 bases per second
- 9. The synthesis of DNA by DNA polymerase occurs in the
Options- A. 3' to 5' direction
- B. 5' to 5' direction
- C. 5' to 3' direction
- D. 3' to 3' direction Discuss
Correct Answer: 5' to 3' direction
- 10. Which of the following statements on replication in E.coli is correct?
Options- A. It occurs in a unidirectional manner
- B. It occurs in bidirectional manner
- C. Always uses T7 DNA polymerase when infected by T7 phage
- D. Occurs only when ? phase has infected E. coli Discuss
Correct Answer: It occurs in bidirectional manner
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