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Microbial Recombination and Gene Transfer problems

  • 1. Which of the following type of recombination does not require homologous sequences and is important for the integration of viral genomes into bacterial chromosomes?

  • Options
  • A. Mutagenic recombimation
  • B. Site-specific recombination
  • C. Replicative recombination
  • D. General recombination
    • Discuss
  • 2. Which of the following is used by microbial genetisists as a tool?

  • Options
  • A. Bacteriophage
  • B. Plasmids
  • C. Transposable elements
  • D. All of these
    • Discuss
  • 3. The expression of gene X (which has promoter Px) is to be monitored. A gene fusion construction for carrying this work will

  • Options
  • A. have Px but not the rest of the X coding region
  • B. have the promoter of lacZ or some other reporter gene
  • C. allow to monitor the expression of all genes with a promoter similar in sequence to Px
  • D. give the same information as from a microarray
    • Discuss
  • 4. The term used for acquisition of naked DNA from its environment and its incorporation in their genome by a bacterium is

  • Options
  • A. transformation
  • B. lysogenic conversion
  • C. conjugation
  • D. transduction
    • Discuss
  • 5. A microarray differs from a gene fusion in that, it

  • Options
  • A. carries DNA segments from many different genes
  • B. is not constructed by cloning
  • C. gives direct measurement of mRNA level
  • D. all of the above
    • Discuss
  • 6. Recombination of virus genomes occurs

  • Options
  • A. by transduction
  • B. by transription
  • C. simultaneous infection of a host cell by two viruses with homologous chromosomes
  • D. by transformation
    • Discuss
  • 7. The plasmids can be eliminated from a cell by the process known as

  • Options
  • A. curing
  • B. breaking
  • C. fixing
  • D. expulsion
    • Discuss
  • 8. R factors involved in plant-microbe interactions are

  • Options
  • A. plant proteins
  • B. bacterial proteins
  • C. essential for transfer of DNA to plant cells
  • D. also called opines
    • Discuss
  • 9. Which of the following statement(s) is/are true in regards to F+ x F- mating events?

  • Options
  • A. DNA is transferred from F- to F+ cells
  • B. DNA is transferred from F+ to F- cells
  • C. No DNA is transferred because F- cells are unable to perform conjugation
  • D. No DNA is transferred because F+ cells are unable to perform conjugation
    • Discuss
  • 10. Penicillin resistance in staphylococci is acquired due to

  • Options
  • A. conjugation
  • B. mutation
  • C. transformation
  • D. transduction
    • Discuss

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