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CuriousTab

DNA Sequencing, Mutation and Repair problems


  • 1. Thymine dimers are directly repaired with the help of visible light by process known as

  • Options
  • A. phosphorylation
  • B. excision repair
  • C. photosynthesis
  • D. photoreactivation
  • Discuss
  • 2. A nonsense mutation may result into

  • Options
  • A. an abnormal elongation of a polypeptide
  • B. a large deletion within the reading frame of a gene
  • C. a premature termination of the synthesis of a polypeptide
  • D. modification of mRNA
  • Discuss
  • 3. The promoter on an expression vector used to overproduce proteins in bacteria is

  • Options
  • A. a bacterial promoter
  • B. expressed both in bacteria and mammalian cells
  • C. not a regulated promoter
  • D. the natural promoter of the gene being cloned
  • Discuss
  • 4. Frame shift mutation may occur as a result of

  • Options
  • A. formation of a thymine-dimer
  • B. deamination of cytosine to uracil
  • C. conversion of guanine to xanthine
  • D. none of the above
  • Discuss
  • 5. Which of the following bacterial genome was sequenced first?

  • Options
  • A. E. coli
  • B. S. pneumoniae
  • C. H. influenzae
  • D. S. thermophilus
  • Discuss
  • 6. What was the first eukaryotic chromosome to be sequenced?

  • Options
  • A. Yeast chromosome III
  • B. Yeast chromosome XI
  • C. Arabidopsis chromosome IV
  • D. None of these
  • Discuss
  • 7. The DNA sequences of rRNA genes can be used to

  • Options
  • A. assess relatedness of eukaryotes but not prokaryotes
  • B. obtain a tentative identification of a new microorganism
  • C. predict the pulsed field gel pattern of a microorganism
  • D. predict whether a bacteria will be gram-positive or gram-negative
  • Discuss
  • 8. The mutation which will not affect the length of a protein is,

  • Options
  • A. nonsense mutation
  • B. missense mutation
  • C. frameshift mutation
  • D. all of these
  • Discuss
  • 9. Due to which of the following DNA repair mechanisms, one is able to distinguish newly synthesized DNA strands from older one?

  • Options
  • A. New strands do not contain cytosine bases
  • B. New strands are lower in molecular size
  • C. Old strands are methylated while new strands are not
  • D. New strands are methylated while old strands are not
  • Discuss
  • 10. Genetic variations are

  • Options
  • A. temporary
  • B. influenced by the environment
  • C. stable
  • D. not heritable
  • Discuss

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