Polymerase Chain Reaction problems
- 1. The cloning step in PCR/sequencing analysis of microbial communities is necessary for
Options- A. the amplification process
- B. preventing contamination by outside DNA
- C. separating the different rRNA gene sequences in the mixture
- D. none of these Discuss
Correct Answer: separating the different rRNA gene sequences in the mixture
- 2. The original enzyme(s) used in PCR reaction is/are known as
Options- A. E. coli DNA polymerase
- B. E. coli RNA polymerase
- C. Taq polymerase
- D. all of these Discuss
Correct Answer: E. coli DNA polymerase
- 3. The template for PCR is
Options- A. RNA
- B. single stranded DNA
- C. double stranded DNA
- D. none of these Discuss
Correct Answer: double stranded DNA
- 4. The efficiency of amplification in PCR in later cycles is reduced due to
Options- A. reduction in substrate concentration
- B. insufficient enzyme and time to synthesize mass quantity of DNA
- C. build up of PCR product which competes with primers for hybrid formation
- D. all of these Discuss
Correct Answer: all of these
- 5. Which of the following infection(s) can be diagnosed by the use of polymerase chain reaction?
Options- A. HIV-1 and HIV-2 viruses
- B. Hepatitis B virus
- C. Mycobacterium tuberculosis
- D. All of these Discuss
Correct Answer: All of these
- 6. The PCR/sequencing approach to rRNA is most likely to give information about
Options- A. the species to which the bacteria are most closely related
- B. morphology of the bacterium
- C. the type of energy metabolism the bacterium has
- D. whether the bacterium is motile Discuss
Correct Answer: the species to which the bacteria are most closely related
- 7. Polymerase chain reaction basically consists of
Options- A. two steps
- B. three steps
- C. four steps
- D. five steps Discuss
Correct Answer: three steps
- 8. RNA populations can also be used as PCR templates after reverse transcription into
Options- A. c DNA
- B. t RNA
- C. m DNA
- D. r RNA Discuss
Correct Answer: c DNA
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