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Biotechnology
‣
Animal Cell Culture and Regulation
Comments
Question
Higher dissolved oxygen concentration in the culture media are toxic and lead to
Options
A. DNA degradation
B. lipid peroxidation
C. metabolism of nutrients in culture media at a rate greater than that required for consumption
D. all of the above
Correct Answer
all of the above
Animal Cell Culture and Regulation problems
Search Results
1. What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?
Options
A. They act as growth promoters
B. They act as growth inhibitors
C. Have no effect on cells
D. Lactate helps in the growth while ammonium inhibits the growth
Show Answer
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Discuss
Correct Answer: Lactate helps in the growth while ammonium inhibits the growth
2. How does CO
2
help in the cell metabolism during cell culture?
Options
A. It participates in the de novo synthesis of purines and pyrimidines
B. Helps in the cells respiration
C. For monitoring pH of the culture
D. All of the above
Show Answer
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Discuss
Correct Answer: It participates in the de novo synthesis of purines and pyrimidines
3. To prevent the accumulation of lactate
Options
A. low glutamine concentration is required
B. high glutamine concentration is required
C. low glucose concentration is required
D. high glucose concentration is required
Show Answer
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Discuss
Correct Answer: low glucose concentration is required
4. Which of the following is the technique used for the embryo culture?
Options
A. Organ cultures on plasma clots
B. Organ cultures on agar
C. Whole embryo cultures
D. All of these
Show Answer
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Discuss
Correct Answer: All of these
5. Accumulation of lactate leads to
Options
A. increase in pH
B. no change in pH
C. reduction in the pH of culture hence loss of cell viability
D. no loss of cell viability
Show Answer
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Discuss
Correct Answer: reduction in the pH of culture hence loss of cell viability
6. pH of culture medium is initially controlled by
Options
A. presence of CO
2
B. presence of bicarbonate buffer
C. addition of bases
D. none of these
Show Answer
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Discuss
Correct Answer: presence of bicarbonate buffer
7. Disaggregating of cells can be achieved by
Options
A. physical disruption
B. enzymatic digestion
C. treating with chelating agents
D. all of the above
Show Answer
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Discuss
Correct Answer: all of the above
8. Some types of variation are due to changes in the genetic material. What is this type of change called?
Options
A. Fertilisation
B. Mutation
C. Radiation
D. Sterilisation
Show Answer
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Discuss
Correct Answer: Mutation
9. Genetic change can occur only in
Options
A. one way
B. two ways
C. three ways
D. four ways
Show Answer
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Discuss
Correct Answer: four ways
10. Animal pharming can be defined as
Options
A. growing animals for farming
B. programming animals to produce novel products
C. generating transgenic animals for farming
D. none of the above
Show Answer
Scratch Pad
Discuss
Correct Answer: programming animals to produce novel products
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