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Home Biotechnology Animal Cell Culture and Regulation Comments

  • Question
  • Accumulation of lactate leads to


  • Options
  • A. increase in pH
  • B. no change in pH
  • C. reduction in the pH of culture hence loss of cell viability
  • D. no loss of cell viability

  • Correct Answer
  • reduction in the pH of culture hence loss of cell viability 


  • Animal Cell Culture and Regulation problems


    Search Results


    • 1. What is the concentration of CO2 required for culturing animal cells?

    • Options
    • A. 2-5%
    • B. 1-10%
    • C. 10-15%
    • D. 15-20%
    • Discuss
    • 2. According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

    • Options
    • A. 6 amino acids
    • B. 8 amino acids
    • C. 10 amino acids
    • D. 12 amino acids
    • Discuss
    • 3. The technique of organ culture may be divided on the basis of employing

    • Options
    • A. solid medium
    • B. liquid medium
    • C. both (a) and (b)
    • D. semi-solid medium
    • Discuss
    • 4. Which of the following is incorrect?

    • Options
    • A. Established cell lines (ECL) have short doubling time
    • B. ECL are invariably aneuploid
    • C. ECL grow in higher density
    • D. ECL do not show much evidence of spatial orientation
    • Discuss
    • 5. Toxicity due to accumulation of ammonia can be overcome

    • Options
    • A. by substituting glutamine by glutamate
    • B. by controlled addition of glutamine at low level
    • C. by removal of ammonia or ammonium from culture medium
    • D. all of the above
    • Discuss
    • 6. Which of the following is the technique used for the embryo culture?

    • Options
    • A. Organ cultures on plasma clots
    • B. Organ cultures on agar
    • C. Whole embryo cultures
    • D. All of these
    • Discuss
    • 7. To prevent the accumulation of lactate

    • Options
    • A. low glutamine concentration is required
    • B. high glutamine concentration is required
    • C. low glucose concentration is required
    • D. high glucose concentration is required
    • Discuss
    • 8. How does CO2 help in the cell metabolism during cell culture?

    • Options
    • A. It participates in the de novo synthesis of purines and pyrimidines
    • B. Helps in the cells respiration
    • C. For monitoring pH of the culture
    • D. All of the above
    • Discuss
    • 9. What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

    • Options
    • A. They act as growth promoters
    • B. They act as growth inhibitors
    • C. Have no effect on cells
    • D. Lactate helps in the growth while ammonium inhibits the growth
    • Discuss
    • 10. Higher dissolved oxygen concentration in the culture media are toxic and lead to

    • Options
    • A. DNA degradation
    • B. lipid peroxidation
    • C. metabolism of nutrients in culture media at a rate greater than that required for consumption
    • D. all of the above
    • Discuss


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