Discussion
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- Question
Toxicity due to accumulation of ammonia can be overcome
Options- A. by substituting glutamine by glutamate
- B. by controlled addition of glutamine at low level
- C. by removal of ammonia or ammonium from culture medium
- D. all of the above
- Correct Answer
- all of the above
- 1. At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via
Options- A. oxidative pathway
- B. anaerobic pathway
- C. both (a) and (b)
- D. none of these Discuss
- 2. Range of optimum glucose concentration present in the culture media is
Options- A. 5.5 - 55 mmol/litre
- B. 55 - 75 mmol/litre
- C. 75-105 mmol/litre
- D. 105-150 mmol/litre Discuss
- 3. Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)
Options- A. 0.05-5 nmol of O2/cell/h
- B. 5 - 10 nmol of O2 /cell/h
- C. 0.05-5 pmol of O2/cell/h
- D. 5 - 10 pmol of O2/cell/h Discuss
- 4. Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is
Options- A. 150-300
- B. 280-360
- C. 300-325
- D. 360-400 Discuss
- 5. Which of the following is not the explantation technique?
Options- A. Slide culture
- B. Carrel flask culture
- C. Roller test tube culture
- D. Adherent primary culture Discuss
- 6. Which of the following is incorrect?
Options- A. Established cell lines (ECL) have short doubling time
- B. ECL are invariably aneuploid
- C. ECL grow in higher density
- D. ECL do not show much evidence of spatial orientation Discuss
- 7. The technique of organ culture may be divided on the basis of employing
Options- A. solid medium
- B. liquid medium
- C. both (a) and (b)
- D. semi-solid medium Discuss
- 8. According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of
Options- A. 6 amino acids
- B. 8 amino acids
- C. 10 amino acids
- D. 12 amino acids Discuss
- 9. What is the concentration of CO2 required for culturing animal cells?
Options- A. 2-5%
- B. 1-10%
- C. 10-15%
- D. 15-20% Discuss
- 10. Accumulation of lactate leads to
Options- A. increase in pH
- B. no change in pH
- C. reduction in the pH of culture hence loss of cell viability
- D. no loss of cell viability Discuss
Animal Cell Culture and Regulation problems
Search Results
Correct Answer: oxidative pathway
Correct Answer: 5.5 - 55 mmol/litre
Correct Answer: 0.05-5 pmol of O2/cell/h
Correct Answer: 280-360
Correct Answer: Adherent primary culture
Correct Answer: ECL grow in higher density
Correct Answer: both (a) and (b)
Correct Answer: 12 amino acids
Correct Answer: 1-10%
Correct Answer: reduction in the pH of culture hence loss of cell viability
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