Discussion
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- Question
Conjugative plasmids
Options- A. exhibit antibiotic resistance
- B. do not exhibit antibiotic resistance
- C. carry transfer genes called the tra genes
- D. do not carry transfer genes
- Correct Answer
- carry transfer genes called the tra genes
- 1. Plasmids which are maintained as limited number of copies per cell are known as
Options- A. stringent plasmids
- B. relaxed plasmids
- C. cryptic plasmids
- D. all of these Discuss
- 2. Maximum size of foreign DNA that can be inserted into an insertion vector is
Options- A. 35 kb
- B. 18 kb
- C. 50 kb
- D. 27 kb Discuss
- 3. Cos site of the cosmids
Options- A. consists of 12 bases
- B. helps whole genome in circularization and ligation
- C. both (a) and (b)
- D. contains cleavage site Discuss
- 4. EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to
Options- A. 6-7 kb
- B. 15-25 kb
- C. 40-44 kb
- D. 1-2 kb Discuss
- 5. Charon vectors are different from EMBL vectors because
Options- A. they have more extensive range of restriction targets with in their polylinkers.
- B. physical separation of lambda arm from central fragment is required
- C. both (a) and (b)
- D. physical separation of lambda arm from central fragment is not required Discuss
- 6. Plasmids which are maintained as multiple copy number per cell are known as
Options- A. stringent plasmids
- B. relaxed plasmids
- C. cryptic plasmids
- D. none of these Discuss
- 7. Maximum size of foreign DNA that can be inserted into a replacement vector is
Options- A. 25-30 kb
- B. 18-20 kb
- C. 20-25 kb
- D. 40-50 kb Discuss
- 8. Select the wrong statement about plasmids?
Options- A. It is extrachromosomal
- B. It is double stranded
- C. Its replication depends upon host cell
- D. It is closed and circular DNA Discuss
- 9. Charon 34 and Charon 35 can clone DNA upto
Options- A. 1-2 kb
- B. 6-7 kb
- C. 9-20 kb
- D. 30-35 kb Discuss
- 10. Cosmid vectors are
Options- A. plasmids that contain fragment of ? DNA including the cos site
- B. phages that lack cos site
- C. plasmids that have no selection marker
- D. cryptic plasmids Discuss
Cloning Vectors problems
Search Results
Correct Answer: stringent plasmids
Correct Answer: 18 kb
Correct Answer: both (a) and (b)
Correct Answer: 15-25 kb
Correct Answer: both (a) and (b)
Correct Answer: relaxed plasmids
Correct Answer: 20-25 kb
Correct Answer: Its replication depends upon host cell
Correct Answer: 9-20 kb
Correct Answer: plasmids that contain fragment of ? DNA including the cos site
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