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- Question
Yeast episomal plasmids (YEp) were constructed using
Options- A. 2?m yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9
- B. 2?m yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322
- C. 12?m yeast plasmid and E coli vector pMB 9
- D. 12?m yeast plasmid and E coli vector pBR 322
- Correct Answer
- 2?m yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9
- 1. Cos cell line is
Options- A. cohesive end site of phage ?
- B. derivative of permissive CV-1 monkey cell line
- C. cohesive initial site of phage ?
- D. derivative of non-permissive CV-1 monkey cell line Discuss
- 2. Recombinant adeno virus can be created by deleting E1A/E 1B genes that are
Options- A. transcriptional regulators and are essential for viral replication
- B. not necessary for viral replication
- C. coding for virus coat protein
- D. important for packaging of viral DNA into viral head Discuss
- 3. Yeast Replicating plasmids (YRp) carry
Options- A. centromere
- B. telomere
- C. autonomously replicating sequence
- D. both (a) and (c) Discuss
- 4. Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?
Options- A. Allows transformation by using crossing over
- B. Has a origin of replication and rep genes
- C. Carries autonomously replicating sequence
- D. Carries centromere and telomere sequences Discuss
- 5. Retroviruses have advantage for being used as vector for animal cells because
Options- A. they cover a wide host range
- B. infection does not lead to cell death, infected cells produce virus over an indefinite period
- C. viral gene expression is driven by strong promoters
- D. all of the above Discuss
- 6. Which one of the following is the most stable vector?
Options- A. YEp
- B. YRp
- C. YAC
- D. YIp Discuss
- 7. SV 40 vectors, in which early region replacements are carried out, require
Options- A. COS cell line
- B. SV 40 helper virus
- C. pBR 322 vector
- D. none of these Discuss
- 8. Which one of the following is true about Yeast Integrative plasmids (YIp) vectors?
Options- A. Allows transformation by using crossing over
- B. Has a origin of replication and rep genes
- C. Carries autonomously replicating sequence
- D. Carries centromere and telomere sequences Discuss
- 9. Which one of the following is true about Yeast Centromeric plasmids (YCp) vectors?
Options- A. Contains centromeric sequences
- B. Segregates during meiosis and mitosis
- C. Functions as true chromosome
- D. All of the above Discuss
- 10. Which of the following can be used for reducing the size of gene inserts containing introns?
Options- A. Vaccinia virus
- B. Baculo virus
- C. Retro virus
- D. none of these Discuss
Vectors Uses for Animal Cell Culture problems
Search Results
Correct Answer: derivative of permissive CV-1 monkey cell line
Correct Answer: transcriptional regulators and are essential for viral replication
Correct Answer: autonomously replicating sequence
Correct Answer: Has a origin of replication and rep genes
Correct Answer: all of the above
Correct Answer: YAC
Correct Answer: COS cell line
Correct Answer: Allows transformation by using crossing over
Correct Answer: All of the above
Correct Answer: Retro virus
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