Discussion
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- Question
Integrated nopaline T-DNA occurs as
Options- A. single segment
- B. two segments
- C. three segments
- D. four segments
- Correct Answer
- single segment
- 1. The left segment of octopine T-DNA (TL) is necessary for
Options- A. enzymes for agropine biosynthesis
- B. tumour formation
- C. conjugative transfer
- D. all of these Discuss
- 2. Liposomes mediated gene transfer in plants involves
Options- A. plasmid DNA enclosed in a lipid bag
- B. fusion of liposomes with protoplast
- C. use of polyethylene glycol (PEG)
- D. all of the above Discuss
- 3. Direct DNA uptake by protoplasts can be stimulated by
Options- A. polyethylene glycol (PEG)
- B. decanal
- C. luciferin
- D. all of these Discuss
- 4. T-DNA transfer and processing into plant genome requires products of which of the following genes?
Options- A. vir A,B
- B. vir G,C
- C. vir D,E
- D. All of these Discuss
- 5. The transfer of intermediate vectors into Agrobacterium are brought about by
Options- A. transformation
- B. biparental mating
- C. triparental mating
- D. transduction Discuss
- 6. In the liposome mediated gene transfer in plants, nucleic acids are
Options- A. protected from nuclease digestion
- B. stable in liposomes
- C. both (a) and (b)
- D. not stable in liposomes Discuss
- 7. Which of the following is not true about the direct repeats flanking T-DNA?
Options- A. They are conserved between nopaline and octopine Ti-plasmids
- B. These repeats are transferred intact to the plant genome
- C. These are important in integration mechanism
- D. all of the above Discuss
- 8. The direct repeats flanking the T-DNA of Agrobacterium tume-faciens are known as
Options- A. cos site
- B. flanking sequences
- C. border sequences
- D. transfer sequences Discuss
- 9. Plant transformation vectors based on Agrobacterium can generally be divided into
Options- A. two vectors
- B. four vectors
- C. six vectors
- D. eight vectors Discuss
- 10. The size of the virulent plasmid of Agrobacterium tumefaciens is
Options- A. 40-80 kb
- B. 80-120 kb
- C. 140-235 kb
- D. >235 kb Discuss
Gene Transfer in Plants problems
Search Results
Correct Answer: tumour formation
Correct Answer: all of the above
Correct Answer: polyethylene glycol (PEG)
Correct Answer: All of these
Correct Answer: triparental mating
Correct Answer: both (a) and (b)
Correct Answer: These repeats are transferred intact to the plant genome
Correct Answer: border sequences
Correct Answer: two vectors
Correct Answer: 140-235 kb
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