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Home Biotechnology Animal Cell Culture and Regulation Comments

  • Question
  • Disaggregating of cells can be achieved by


  • Options
  • A. physical disruption
  • B. enzymatic digestion
  • C. treating with chelating agents
  • D. all of the above

  • Correct Answer
  • all of the above 


  • Animal Cell Culture and Regulation problems


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    • 1. pH of culture medium is initially controlled by

    • Options
    • A. presence of CO2
    • B. presence of bicarbonate buffer
    • C. addition of bases
    • D. none of these
    • Discuss
    • 2. Higher dissolved oxygen concentration in the culture media are toxic and lead to

    • Options
    • A. DNA degradation
    • B. lipid peroxidation
    • C. metabolism of nutrients in culture media at a rate greater than that required for consumption
    • D. all of the above
    • Discuss
    • 3. What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

    • Options
    • A. They act as growth promoters
    • B. They act as growth inhibitors
    • C. Have no effect on cells
    • D. Lactate helps in the growth while ammonium inhibits the growth
    • Discuss
    • 4. How does CO2 help in the cell metabolism during cell culture?

    • Options
    • A. It participates in the de novo synthesis of purines and pyrimidines
    • B. Helps in the cells respiration
    • C. For monitoring pH of the culture
    • D. All of the above
    • Discuss
    • 5. To prevent the accumulation of lactate

    • Options
    • A. low glutamine concentration is required
    • B. high glutamine concentration is required
    • C. low glucose concentration is required
    • D. high glucose concentration is required
    • Discuss
    • 6. Some types of variation are due to changes in the genetic material. What is this type of change called?

    • Options
    • A. Fertilisation
    • B. Mutation
    • C. Radiation
    • D. Sterilisation
    • Discuss
    • 7. Genetic change can occur only in

    • Options
    • A. one way
    • B. two ways
    • C. three ways
    • D. four ways
    • Discuss
    • 8. Animal pharming can be defined as

    • Options
    • A. growing animals for farming
    • B. programming animals to produce novel products
    • C. generating transgenic animals for farming
    • D. none of the above
    • Discuss
    • 9. Which of the following statement is correct?

    • Options
    • A. Variation is caused by genes
    • B. Variation is caused by the environment
    • C. Variation can be caused by both genes and the environment
    • D. None of the above
    • Discuss
    • 10. Which of the following is/are the method of transfection for making transgenic animals?

    • Options
    • A. Transfer of whole nuclei
    • B. Transfer of whole individual chromosomes or fragment
    • C. Transfer of DNA
    • D. All of the above
    • Discuss


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