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Biotechnology
‣
Animal Cell Culture and Regulation
Comments
Question
Disaggregating of cells can be achieved by
Options
A. physical disruption
B. enzymatic digestion
C. treating with chelating agents
D. all of the above
Correct Answer
all of the above
Animal Cell Culture and Regulation problems
Search Results
1. pH of culture medium is initially controlled by
Options
A. presence of CO
2
B. presence of bicarbonate buffer
C. addition of bases
D. none of these
Show Answer
Scratch Pad
Discuss
Correct Answer: presence of bicarbonate buffer
2. Higher dissolved oxygen concentration in the culture media are toxic and lead to
Options
A. DNA degradation
B. lipid peroxidation
C. metabolism of nutrients in culture media at a rate greater than that required for consumption
D. all of the above
Show Answer
Scratch Pad
Discuss
Correct Answer: all of the above
3. What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?
Options
A. They act as growth promoters
B. They act as growth inhibitors
C. Have no effect on cells
D. Lactate helps in the growth while ammonium inhibits the growth
Show Answer
Scratch Pad
Discuss
Correct Answer: Lactate helps in the growth while ammonium inhibits the growth
4. How does CO
2
help in the cell metabolism during cell culture?
Options
A. It participates in the de novo synthesis of purines and pyrimidines
B. Helps in the cells respiration
C. For monitoring pH of the culture
D. All of the above
Show Answer
Scratch Pad
Discuss
Correct Answer: It participates in the de novo synthesis of purines and pyrimidines
5. To prevent the accumulation of lactate
Options
A. low glutamine concentration is required
B. high glutamine concentration is required
C. low glucose concentration is required
D. high glucose concentration is required
Show Answer
Scratch Pad
Discuss
Correct Answer: low glucose concentration is required
6. Some types of variation are due to changes in the genetic material. What is this type of change called?
Options
A. Fertilisation
B. Mutation
C. Radiation
D. Sterilisation
Show Answer
Scratch Pad
Discuss
Correct Answer: Mutation
7. Genetic change can occur only in
Options
A. one way
B. two ways
C. three ways
D. four ways
Show Answer
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Discuss
Correct Answer: four ways
8. Animal pharming can be defined as
Options
A. growing animals for farming
B. programming animals to produce novel products
C. generating transgenic animals for farming
D. none of the above
Show Answer
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Discuss
Correct Answer: programming animals to produce novel products
9. Which of the following statement is correct?
Options
A. Variation is caused by genes
B. Variation is caused by the environment
C. Variation can be caused by both genes and the environment
D. None of the above
Show Answer
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Discuss
Correct Answer: Variation is caused by genes
10. Which of the following is/are the method of transfection for making transgenic animals?
Options
A. Transfer of whole nuclei
B. Transfer of whole individual chromosomes or fragment
C. Transfer of DNA
D. All of the above
Show Answer
Scratch Pad
Discuss
Correct Answer: All of the above
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