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- Question
How does CO2 help in the cell metabolism during cell culture?
Options- A. It participates in the de novo synthesis of purines and pyrimidines
- B. Helps in the cells respiration
- C. For monitoring pH of the culture
- D. All of the above
- Correct Answer
- It participates in the de novo synthesis of purines and pyrimidines
- 1. To prevent the accumulation of lactate
Options- A. low glutamine concentration is required
- B. high glutamine concentration is required
- C. low glucose concentration is required
- D. high glucose concentration is required Discuss
- 2. Which of the following is the technique used for the embryo culture?
Options- A. Organ cultures on plasma clots
- B. Organ cultures on agar
- C. Whole embryo cultures
- D. All of these Discuss
- 3. Accumulation of lactate leads to
Options- A. increase in pH
- B. no change in pH
- C. reduction in the pH of culture hence loss of cell viability
- D. no loss of cell viability Discuss
- 4. What is the concentration of CO2 required for culturing animal cells?
Options- A. 2-5%
- B. 1-10%
- C. 10-15%
- D. 15-20% Discuss
- 5. According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of
Options- A. 6 amino acids
- B. 8 amino acids
- C. 10 amino acids
- D. 12 amino acids Discuss
- 6. What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?
Options- A. They act as growth promoters
- B. They act as growth inhibitors
- C. Have no effect on cells
- D. Lactate helps in the growth while ammonium inhibits the growth Discuss
- 7. Higher dissolved oxygen concentration in the culture media are toxic and lead to
Options- A. DNA degradation
- B. lipid peroxidation
- C. metabolism of nutrients in culture media at a rate greater than that required for consumption
- D. all of the above Discuss
- 8. pH of culture medium is initially controlled by
Options- A. presence of CO2
- B. presence of bicarbonate buffer
- C. addition of bases
- D. none of these Discuss
- 9. Disaggregating of cells can be achieved by
Options- A. physical disruption
- B. enzymatic digestion
- C. treating with chelating agents
- D. all of the above Discuss
- 10. Some types of variation are due to changes in the genetic material. What is this type of change called?
Options- A. Fertilisation
- B. Mutation
- C. Radiation
- D. Sterilisation Discuss
Animal Cell Culture and Regulation problems
Search Results
Correct Answer: low glucose concentration is required
Correct Answer: All of these
Correct Answer: reduction in the pH of culture hence loss of cell viability
Correct Answer: 1-10%
Correct Answer: 12 amino acids
Correct Answer: Lactate helps in the growth while ammonium inhibits the growth
Correct Answer: all of the above
Correct Answer: presence of bicarbonate buffer
Correct Answer: all of the above
Correct Answer: Mutation
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