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Home Biotechnology Animal Cell Culture and Regulation Comments

  • Question
  • Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is


  • Options
  • A. 150-300
  • B. 280-360
  • C. 300-325
  • D. 360-400

  • Correct Answer
  • 280-360 


  • Animal Cell Culture and Regulation problems


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    • 1. Which of the following is not the explantation technique?

    • Options
    • A. Slide culture
    • B. Carrel flask culture
    • C. Roller test tube culture
    • D. Adherent primary culture
    • Discuss
    • 2. An established cell line can be called where it has been subcultured at least

    • Options
    • A. 70 times at an interval of 3 days between subcultures
    • B. 40 times at an interval of 3 days between subcultures
    • C. 70 times at an interval of 1 day between subcultures
    • D. 50 times at an interval of 3 days between subcultures
    • Discuss
    • 3. Which of the followings are the metabolic products of glucose and glutamine?

    • Options
    • A. CO2 and NH3
    • B. CO2 and lactate
    • C. Lactate and ammonium
    • D. Lactate only
    • Discuss
    • 4. In animal cell culture, particularly mammalian cell culture, transformation means

    • Options
    • A. uptake of new genetic material
    • B. phenotypic modifications of cells in culture
    • C. both (a) and (b)
    • D. release of genetic information
    • Discuss
    • 5. For culturing, plasma from the adult chicken is preferred to mammalian plasma because

    • Options
    • A. it forms a clear and solid coagulum even after dilution
    • B. it is too opaque
    • C. it doesn't produce solid clots
    • D. it forms a semi solid coagulum
    • Discuss
    • 6. Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

    • Options
    • A. 0.05-5 nmol of O2/cell/h
    • B. 5 - 10 nmol of O2 /cell/h
    • C. 0.05-5 pmol of O2/cell/h
    • D. 5 - 10 pmol of O2/cell/h
    • Discuss
    • 7. Range of optimum glucose concentration present in the culture media is

    • Options
    • A. 5.5 - 55 mmol/litre
    • B. 55 - 75 mmol/litre
    • C. 75-105 mmol/litre
    • D. 105-150 mmol/litre
    • Discuss
    • 8. At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

    • Options
    • A. oxidative pathway
    • B. anaerobic pathway
    • C. both (a) and (b)
    • D. none of these
    • Discuss
    • 9. Toxicity due to accumulation of ammonia can be overcome

    • Options
    • A. by substituting glutamine by glutamate
    • B. by controlled addition of glutamine at low level
    • C. by removal of ammonia or ammonium from culture medium
    • D. all of the above
    • Discuss
    • 10. Which of the following is incorrect?

    • Options
    • A. Established cell lines (ECL) have short doubling time
    • B. ECL are invariably aneuploid
    • C. ECL grow in higher density
    • D. ECL do not show much evidence of spatial orientation
    • Discuss


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