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- Question
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is
Options- A. 150-300
- B. 280-360
- C. 300-325
- D. 360-400
- Correct Answer
- 280-360
- 1. Which of the following is not the explantation technique?
Options- A. Slide culture
- B. Carrel flask culture
- C. Roller test tube culture
- D. Adherent primary culture Discuss
- 2. An established cell line can be called where it has been subcultured at least
Options- A. 70 times at an interval of 3 days between subcultures
- B. 40 times at an interval of 3 days between subcultures
- C. 70 times at an interval of 1 day between subcultures
- D. 50 times at an interval of 3 days between subcultures Discuss
- 3. Which of the followings are the metabolic products of glucose and glutamine?
Options- A. CO2 and NH3
- B. CO2 and lactate
- C. Lactate and ammonium
- D. Lactate only Discuss
- 4. In animal cell culture, particularly mammalian cell culture, transformation means
Options- A. uptake of new genetic material
- B. phenotypic modifications of cells in culture
- C. both (a) and (b)
- D. release of genetic information Discuss
- 5. For culturing, plasma from the adult chicken is preferred to mammalian plasma because
Options- A. it forms a clear and solid coagulum even after dilution
- B. it is too opaque
- C. it doesn't produce solid clots
- D. it forms a semi solid coagulum Discuss
- 6. Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)
Options- A. 0.05-5 nmol of O2/cell/h
- B. 5 - 10 nmol of O2 /cell/h
- C. 0.05-5 pmol of O2/cell/h
- D. 5 - 10 pmol of O2/cell/h Discuss
- 7. Range of optimum glucose concentration present in the culture media is
Options- A. 5.5 - 55 mmol/litre
- B. 55 - 75 mmol/litre
- C. 75-105 mmol/litre
- D. 105-150 mmol/litre Discuss
- 8. At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via
Options- A. oxidative pathway
- B. anaerobic pathway
- C. both (a) and (b)
- D. none of these Discuss
- 9. Toxicity due to accumulation of ammonia can be overcome
Options- A. by substituting glutamine by glutamate
- B. by controlled addition of glutamine at low level
- C. by removal of ammonia or ammonium from culture medium
- D. all of the above Discuss
- 10. Which of the following is incorrect?
Options- A. Established cell lines (ECL) have short doubling time
- B. ECL are invariably aneuploid
- C. ECL grow in higher density
- D. ECL do not show much evidence of spatial orientation Discuss
Animal Cell Culture and Regulation problems
Search Results
Correct Answer: Adherent primary culture
Correct Answer: 70 times at an interval of 3 days between subcultures
Correct Answer: Lactate and ammonium
Correct Answer: phenotypic modifications of cells in culture
Correct Answer: it forms a clear and solid coagulum even after dilution
Correct Answer: 0.05-5 pmol of O2/cell/h
Correct Answer: 5.5 - 55 mmol/litre
Correct Answer: oxidative pathway
Correct Answer: all of the above
Correct Answer: ECL grow in higher density
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