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Biotechnology
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Animal Cell Culture and Regulation
Comments
Question
For culturing, plasma from the adult chicken is preferred to mammalian plasma because
Options
A. it forms a clear and solid coagulum even after dilution
B. it is too opaque
C. it doesn't produce solid clots
D. it forms a semi solid coagulum
Correct Answer
it forms a clear and solid coagulum even after dilution
Animal Cell Culture and Regulation problems
Search Results
1. Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping?
Options
A. Down's syndrome
B. Turner's syndrome
C. Klinefelter's syndrome
D. all of these
Show Answer
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Discuss
Correct Answer: all of these
2. Cells which have undergone transformation frequently become
Options
A. anchorage independent
B. anchorage dependent
C. stable
D. unstable
Show Answer
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Discuss
Correct Answer: anchorage independent
3. During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture?
Options
A. Ethyl alcohol is being produced in excess
B. The cells have too much oxygen
C. Glycolysis is being inhibited
D. The cells do not have enough oxygen
Show Answer
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Correct Answer: The cells do not have enough oxygen
4. When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of
Options
A. incomplete glutamine oxidation
B. increase in specific lactate production from glucose
C. both (a) and (b)
D. accumulation of ammonia
Show Answer
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Correct Answer: both (a) and (b)
5. The human fibroplast is a classical example of
Options
A. stable primary cell lines
B. established cell lines
C. cell transformations
D. none of these
Show Answer
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Correct Answer: stable primary cell lines
6. In animal cell culture, particularly mammalian cell culture, transformation means
Options
A. uptake of new genetic material
B. phenotypic modifications of cells in culture
C. both (a) and (b)
D. release of genetic information
Show Answer
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Correct Answer: phenotypic modifications of cells in culture
7. Which of the followings are the metabolic products of glucose and glutamine?
Options
A. CO
2
and NH
3
B. CO
2
and lactate
C. Lactate and ammonium
D. Lactate only
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Correct Answer: Lactate and ammonium
8. An established cell line can be called where it has been subcultured at least
Options
A. 70 times at an interval of 3 days between subcultures
B. 40 times at an interval of 3 days between subcultures
C. 70 times at an interval of 1 day between subcultures
D. 50 times at an interval of 3 days between subcultures
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Correct Answer: 70 times at an interval of 3 days between subcultures
9. Which of the following is not the explantation technique?
Options
A. Slide culture
B. Carrel flask culture
C. Roller test tube culture
D. Adherent primary culture
Show Answer
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Correct Answer: Adherent primary culture
10. Range of osmolarity tolerated/accepted in mOsm/Kg of H
2
O by mammalian cells is
Options
A. 150-300
B. 280-360
C. 300-325
D. 360-400
Show Answer
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Discuss
Correct Answer: 280-360
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