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Biotechnology
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Animal Cell Culture and Regulation
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Question
What are the main constituents of culture for animal cell growth?
Options
A. Glucose and Glutamine
B. Growth factors
C. Cytokines
D. All of these
Correct Answer
Glucose and Glutamine
Animal Cell Culture and Regulation problems
Search Results
1. The major problem associated with the isolation of free cells and cell aggregates from organs is that of
Options
A. releasing the cells from their supporting matrix
B. inhibiting the cells from their supporting matrix
C. disintegrating the cells from their supporting matrix
D. none of the above
Show Answer
Scratch Pad
Discuss
Correct Answer: releasing the cells from their supporting matrix
2. Which of the following is correct?
Options
A. ECL can be established in suspension cultures whereas it is exceptional for primary cell lines (PCL)
B. ECL and PCL can be established in suspension cultures
C. PCL can be established in suspension cultures
D. none of the above
Show Answer
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Discuss
Correct Answer: ECL can be established in suspension cultures whereas it is exceptional for primary cell lines (PCL)
3. Excess CO
2
suppress cell growth and productivity by
Options
A. inhibiting respiration
B. altering intracellular pH by diffusing across cell membrane
C. both (a) and (b)
D. altering pH of the medium
Show Answer
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Discuss
Correct Answer: both (a) and (b)
4. Klinefelter syndrome is a manifestation of which chromosome aneuploidy?
Options
A. XXX
B. XYY
C. XXY
D. None of these
Show Answer
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Discuss
Correct Answer: XXY
5. Which of the following sets belongs to a distinct class or category?
Options
A. Ribose, sucrose, glucose, fructose
B. Sterols, waxes, fatty acids, amino acids.
C. Oils, sterols, waxes, fats
D. Nucleotide, nucleoside, nucleus, DNA
Show Answer
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Discuss
Correct Answer: Sterols, waxes, fatty acids, amino acids.
6. Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called
Options
A. established cell lines
B. primary cell lines
C. secondary cell lines
D. propagated cell lines
Show Answer
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Discuss
Correct Answer: established cell lines
7. Range of optimum glutamine concentration present in the culture media is
Options
A. 1-2 mmol/litre
B. 2-7 mmol/litre
C. 7-15 mmol/litre
D. 15 - 20 mmol/litre
Show Answer
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Discuss
Correct Answer: 2-7 mmol/litre
8. Which of the following is not a source of energy in active muscle cells?
Options
A. Creatine phosphate
B. ATP
C. Lactic acid
D. Glucose
Show Answer
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Discuss
Correct Answer: Lactic acid
9. The human fibroplast is a classical example of
Options
A. stable primary cell lines
B. established cell lines
C. cell transformations
D. none of these
Show Answer
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Discuss
Correct Answer: stable primary cell lines
10. When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of
Options
A. incomplete glutamine oxidation
B. increase in specific lactate production from glucose
C. both (a) and (b)
D. accumulation of ammonia
Show Answer
Scratch Pad
Discuss
Correct Answer: both (a) and (b)
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