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Media Required for Plant Cell Culture
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Question
Which of the following is not true about nurse or conditioned medium?
Options
A. It is liquid removed from the suspension of fast growing cells
B. It contains uncharacterized growth factor released by growing cells
C. It is used in the culture of regenerating protoplast
D. it is removed aseptically from the culture and is autoclaved before use
Correct Answer
it is removed aseptically from the culture and is autoclaved before use
Media Required for Plant Cell Culture problems
Search Results
1. Concentration of sucrose generally used in plant cell culture media is
Options
A. 10-15 g/l
B. 20-30 g/l
C. 40-50 g/l
D. 60-70 g/l
Show Answer
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Correct Answer: 20-30 g/l
2. Which of the following is an ethylene biosynthesis inhibitor?
Options
A. Citric acid
B. Succinic acid
C. Activated charcoal
D. Silver thiosulphate
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Correct Answer: Silver thiosulphate
3. Which is the most common carbon source used in the plant cell culture media?
Options
A. Sucrose
B. Glucose
C. Fructose
D. Maltose
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Correct Answer: Sucrose
4. Which of the following is not an auxin?
Options
A. Indole acetic acid (IAA)
B. Naphthalenacetic acid (NAA)
C. zeatin
D. Indole butyric acid
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Correct Answer: zeatin
5. Which of the following growth regulator promote cell division?
Options
A. Auxins
B. Cytokinins
C. Gibberellins
D. Brassinosteroids
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Correct Answer: Cytokinins
6. Which of the following growth regulator is used to stimulate embryo or shoot development?
Options
A. Auxins
B. Cytokinins
C. Gibberellins
D. Brassinosteroids
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Correct Answer: Gibberellins
7. To maintain the pH of the culture
Options
A. organic acid such as citric, fumaric, malic and succinic acid is used
B. synthetic buffers such as Tris, MES or HEPS are used
C. both (a) and (b)
D. ammonium salts are used
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Correct Answer: both (a) and (b)
8. Starch content of potatoes can be increased by using a bacterial gene, known as
Options
A. sucrose phosphate synthase gene
B. ADP glucose pyrophosphorylase gene
C. polygalactouranase gene
D. none of the above
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Correct Answer: ADP glucose pyrophosphorylase gene
9. What are the various disadvantages of cross protection?
Options
A. Possibility of mutations in inducing mild virus strain
B. Possibility of synergism between inducing virus and other unrelated virus
C. Possibility of unnecessary spread of mild virus
D. All of the above
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Correct Answer: All of the above
10. Insect resistance in the transgenic plant has been achieved by
Options
A. transferring genes for Bt toxins
B. transferring genes for protease inhibitors
C. transferring genes for other insecticidal secondary metabolities
D. all of the above
Show Answer
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Correct Answer: all of the above
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